Supervillin Antibody (6E10)

Novus Biologicals, part of Bio-Techne | Catalog # H00006840-M03

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 6E10

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Supervillin Antibodies »

Product Specifications

Immunogen

SVIL (NP_003165, 1679 a.a. ~ 1786 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. LIHAGLEPLTFTNMFPSWEHREDIAEITEMDTEVSNQITLVEDVLAKLCKTIYPLADLLARPLPEGVDPLKLEIYLTDEDFEFALDMTRDEYNALPAWKQVNLKKAKG

Specificity

SVIL - supervillin

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Supervillin Antibody (6E10)

Immunocytochemistry/ Immunofluorescence: Supervillin Antibody (6E10) [H00006840-M03]

Immunocytochemistry/ Immunofluorescence: Supervillin Antibody (6E10) [H00006840-M03]

Immunocytochemistry/Immunofluorescence: Supervillin Antibody (6E10) [H00006840-M03] - Analysis of monoclonal antibody to SVIL on HeLa cell. Antibody concentration 10 ug/ml
Sandwich ELISA: Supervillin Antibody (6E10) [H00006840-M03] - Detection limit for recombinant GST tagged SVIL is approximately 3ng/ml as a capture antibody.

Applications for Supervillin Antibody (6E10)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Supervillin

This gene encodes a bipartite protein with distinct amino- and carboxy-terminal domains. The amino-terminus contains nuclear localization signals and the carboxy-terminus contains numerous consecutive sequences with extensive similarity to proteins in the gelsolin family of actin-binding proteins, which cap, nucleate, and/or sever actin filaments. The gene product is tightly associated with both actin filaments and plasma membranes, suggesting a role as a high-affinity link between the actin cytoskeleton and the membrane. The encoded protein appears to aid in both myosin II assembly during cell spreading and disassembly of focal adhesions. Two transcript variants encoding different isoforms of supervillin have been described.

Alternate Names

Archvillin, p205/p250, SVIL

Entrez Gene IDs

6840 (Human)

Gene Symbol

SVIL

OMIM

604126 (Human)

UniProt

NP_003165 (Human)

Additional Supervillin Products

Product Documents for Supervillin Antibody (6E10)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Supervillin Antibody (6E10)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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