Supervillin Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-90363

Novus Biologicals, part of Bio-Techne
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NBP1-90363
NBP1-90363-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Supervillin Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: NARYQTQPVTLGEVEQVQSGKLIPFSPAVNTSVSTVASTVAPMYAGDLRTKPPLDHNASATDYKFSSSIENSDSPVRSILKSQAWQPLVEGSENKGMLREYGETESKRALTGRDSGMEKYGSFEEAEASYPILNRARE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Supervillin Antibody

Western Blot: Supervillin Antibody [NBP1-90363]

Western Blot: Supervillin Antibody [NBP1-90363]

Western Blot: Supervillin Antibody [NBP1-90363] - Analysis in human cell lines A-431 and PC-3 using anti-SVIL antibody. Corresponding SVIL RNA-seq data are presented for the same cell lines. Loading control: anti-PPIB.
Immunocytochemistry/ Immunofluorescence: Supervillin Antibody [NBP1-90363]

Immunocytochemistry/ Immunofluorescence: Supervillin Antibody [NBP1-90363]

Immunocytochemistry/Immunofluorescence: Supervillin Antibody [NBP1-90363] - Staining of human cell line A-431 shows localization to plasma membrane, cytosol & actin filaments. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Supervillin Antibody [NBP1-90363]

Immunohistochemistry-Paraffin: Supervillin Antibody [NBP1-90363]

Immunohistochemistry-Paraffin: Supervillin Antibody [NBP1-90363] - Staining of human heart muscle shows moderate cytoplasmic positivity in cardiomyocytes.
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Applications for Supervillin Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Supervillin

SVIL - supervillin

Alternate Names

Archvillin, p205/p250, SVIL

Gene Symbol

SVIL

Additional Supervillin Products

Product Documents for Supervillin Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Supervillin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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