Suppressor of Fused Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-35342

Novus Biologicals, part of Bio-Techne
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NBP3-35342-100ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Suppressor of Fused Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 325-484 of human Suppressor of Fused (NP_057253.2).

Sequence:
PPINPQRQNGLAHDRAPSRKDSLESDSSTAIIPHELIRTRQLESVHLKFNQESGALIPLCLRGRLLHGRHFTYKSITGDMAITFVSTGVEGAFATEEHPYAAHGPWLQILLTEEFVEKMLEDLEDLTSPEEFKLPKEYSWPEKKLKVSILPDVVFDSPLH

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

54 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Suppressor of Fused Antibody

Suppressor of Fused Antibody

Western Blot: Suppressor of Fused Antibody [NBP3-35342] -

Western Blot: Suppressor of Fused Antibody [NBP3-35342] - Western blot analysis of lysates from Mouse testis using Suppressor of Fused Rabbit pAb at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25 ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Suppressor of Fused Antibody

Immunocytochemistry/ Immunofluorescence: Suppressor of Fused Antibody [NBP3-35342] -

Immunocytochemistry/ Immunofluorescence: Suppressor of Fused Antibody [NBP3-35342] - Immunofluorescence analysis of MCF-7 cells using Suppressor of Fused Rabbit pAb. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
Suppressor of Fused Antibody

Immunohistochemistry: Suppressor of Fused Antibody [NBP3-35342] -

Immunohistochemistry: Suppressor of Fused Antibody [NBP3-35342] - Immunohistochemistry analysis of paraffin-embedded Human esophageal cancer using Suppressor of Fused Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
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Applications for Suppressor of Fused Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Suppressor of Fused

The Hedgehog (Hh) signaling pathway has conserved roles in development of species ranging from Drosophila to humans. Human supressor of fused HSu(Fu) acts as a negative regulator in the hedgehog signaling (SHH) pathway by down-regulating GLI1-mediated transactivation of target genes (1). HSu(fu) was found to repress activity of the zinc-finger transcription factor Gli, which mediates Hedgehog signaling in vertebrates, and to physically interact with Gli, Gli2 and Gli3 (2). The sonic hedgehog signaling pathway directs the embryonic development of diverse organisms and is disrupted in a variety of malignancies. Several of mutations encode truncated proteins that are unable to export the GLI transcription factor from nucleus to cytoplasm, resulting in the activation of SHH signaling. SUFU is a newly identified tumor-suppressor gene that predisposes individuals to medulloblastoma by modulating the SHH signaling pathway through a newly identified mechanism (3).

Alternate Names

SUFUHSUFUXLPRO1280, suppressor of fused homolog, suppressor of fused homolog (Drosophila)

Gene Symbol

SUFU

Additional Suppressor of Fused Products

Product Documents for Suppressor of Fused Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Suppressor of Fused Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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