SUPT16H Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-60942

Novus Biologicals, part of Bio-Techne
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NB100-60942
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Bovine (100%), Mouse (100%), Porcine (100%). Backed by our 100% Guarantee.

Applications

Validated:

Peptide ELISA, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all SUPT16H Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-DLTNKEGKKPEEK corresponding to internal region according to NP_009123.1.

Epitope

DLTNKEGKKPEEK

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for SUPT16H Antibody

Western Blot: SUPT16H Antibody [NB100-60942]

Western Blot: SUPT16H Antibody [NB100-60942]

Western Blot: SUPT16H Antibody [NB100-60942] - (0.1ug/ml) staining of HeLa cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for SUPT16H Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:8000

Western Blot

0.1-0.3 ug/ml
Application Notes
WB: Approx. 150 kDa band observed in HeLa lysates (calculated MW of 120 kDa band according to NP_009123.1). An additional band of unknown identity was also consistently observed at 30 kDa. This band was successfully blocked by incubation with the immunizing peptide.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SUPT16H

Transcription of protein-coding genes can be reconstituted on naked DNA with only the general transcription factors and RNA polymerase II. However, this minimal system cannot transcribe DNA packaged into chromatin, indicating that accessory factors may facilitate access to DNA. One such factor, FACT (facilitates chromatin transcription), interacts specifically with histones H2A/H2B to effect nucleosome disassembly and transcription elongation. FACT is composed of an 80 kDa subunit and a 140 kDa subunit, the latter of which is the protein encoded by this gene.

Alternate Names

CDC68, Chromatin-specific transcription elongation factor 140 kDa subunit, facilitates chromatin remodeling 140 kDa subunit, Facilitates chromatin transcription complex subunit SPT16, FACT 140 kDa subunit, FACT complex subunit SPT16, FACT140, FACTp140, FACTP140FACT, FLJ10857, FLJ14010, FLJ34357, hSPT16, SPT16/CDC68, suppressor of Ty (S.cerevisiae) 16 homolog, suppressor of Ty 16 homolog (S. cerevisiae)

Entrez Gene IDs

11198 (Human)

Gene Symbol

SUPT16H

UniProt

NP_009123.1 (Human)

Additional SUPT16H Products

Product Documents for SUPT16H Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for SUPT16H Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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