SUPT5H Antibody (9V9G7)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-33195
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rabbit IgG Clone # 9V9G7
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 800-900 of human SUPT5H (O00267).
Sequence:
PHYGSQTPLHDGSRTPAQSGAWDPNNPNTPSRAEEEYEYAFDDEPTPSPQAYGGTPNPQTPGYPDPSSPQVNPQYNPQTPGTPAMYNTDQFSPYAAPSPQG
Sequence:
PHYGSQTPLHDGSRTPAQSGAWDPNNPNTPSRAEEEYEYAFDDEPTPSPQAYGGTPNPQTPGYPDPSSPQVNPQYNPQTPGTPAMYNTDQFSPYAAPSPQG
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
121 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for SUPT5H Antibody (9V9G7)
Western Blot: SUPT5H Antibody (9V9G7) [NBP3-33195] -
Western Blot: SUPT5H Antibody (9V9G7) [NBP3-33195] - Western blot analysis of lysates from HeLa cells, using SUPT5H Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
Immunohistochemistry: SUPT5H Antibody (9V9G7) [NBP3-33195] -
Immunohistochemistry: SUPT5H Antibody (9V9G7) [NBP3-33195] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using SUPT5H Rabbit mAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
Immunocytochemistry/ Immunofluorescence: SUPT5H Antibody (9V9G7) [NBP3-33195] -
Immunocytochemistry/ Immunofluorescence: SUPT5H Antibody (9V9G7) [NBP3-33195] - Immunofluorescence analysis of NIH/3T3 cells using SUPT5H Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
Applications for SUPT5H Antibody (9V9G7)
Application
Recommended Usage
ELISA
Recommended starting concentration is 1 ug/mL
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Immunohistochemistry-Paraffin
1:50 - 1:200
Western Blot
1:500 - 1:1000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: SUPT5H
Alternate Names
DRB sensitivity-inducing factor 160 kDa subunit, DRB sensitivity-inducing factor large subunit, DSIF large subunit, DSIF p160, FLJ34157, SPT5hSPT5, SPT5HTat-cotransactivator 1 protein, suppressor of Ty (S.cerevisiae) 5 homolog, suppressor of Ty 5 homolog (S. cerevisiae), Tat-CT1, Tat-CT1 protein, transcription elongation factor SPT5
Gene Symbol
SUPT5H
Additional SUPT5H Products
Product Documents for SUPT5H Antibody (9V9G7)
Product Specific Notices for SUPT5H Antibody (9V9G7)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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