SURF5 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-87818

Novus Biologicals, part of Bio-Techne
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NBP1-87818
NBP1-87818-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all SURF5 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: MAQQRALPQSKETLLQSYNKRLKDDIKSIMDNFTEIIKTAKIEDETQVSRATQGEQDNYEMHVRAANIVRAGESLMKLVSDLKQFLILNDFPSVNEAIDQRN

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SURF5 Antibody

Western Blot: SURF5 Antibody [NBP1-87818]

Western Blot: SURF5 Antibody [NBP1-87818]

Western Blot: SURF5 Antibody [NBP1-87818] - Analysis in control (vector only transfected HEK293T lysate) and MED22 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: SURF5 Antibody [NBP1-87818]

Immunohistochemistry-Paraffin: SURF5 Antibody [NBP1-87818]

Immunohistochemistry-Paraffin: SURF5 Antibody [NBP1-87818] - Staining of human kidney shows strong membranous positivity in renal glomeruli.

Applications for SURF5 Antibody

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SURF5

SURF5 is located in the surfeit gene cluster, a group of very tightly linked housekeeping genes that do not share sequence similarity. The gene is oriented in a head-to-head fashion with RPL7A (SURF3) and the two genes share a bidirectional promoter. The encoded proteins are localized to the cytoplasm. Two alternative transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq]

Alternate Names

Med24, mediator complex subunit 22SURF5surfeit 5, mediator of RNA polymerase II transcription subunit 22, MGC48682, Surf-5, Surfeit locus protein 5

Gene Symbol

MED22

Additional SURF5 Products

Product Documents for SURF5 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for SURF5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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