Survivin Antibody (60.11) - Unpurified

Novus Biologicals, part of Bio-Techne | Catalog # NB500-205

Novus Biologicals, part of Bio-Techne
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NB500-205
NB500-205SS
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse

Applications

Validated:

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Knockdown Validated, Proximity Ligation Assay, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Proximity Ligation Assay, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 60.11

Format

Unpurified

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all Survivin Antibodies »

Product Specifications

Immunogen

This Survivin Antibody (60.11) was developed against full length recombinant human Survivin [UniProt# O15392].

Epitope

The epitope recognized is between amino acids 57-67.

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 30572639)

Localization

Cytoplasmic

Specificity

Survivin Antibody (60.11) [NB500-205] is specific for the cytoplasmic form of survivin.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Theoretical MW

16 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Survivin Antibody (60.11) - Unpurified

Western Blot: Survivin Antibody (60.11)Unpurified [NB500-205]

Western Blot: Survivin Antibody (60.11)Unpurified [NB500-205]

Western Blot: Survivin Antibody (60.11) - Unpurified [NB500-205] - Survivin detected in rat aorta smooth muscle cell lysate in western blot analysis using [NB500-205]. Theoretical molecular weight: 16 kDa.
Immunocytochemistry/ Immunofluorescence: Survivin Antibody (60.11) - Unpurified [NB500-205]

Immunocytochemistry/ Immunofluorescence: Survivin Antibody (60.11) - Unpurified [NB500-205]

Immunocytochemistry/Immunofluorescence: Survivin Antibody (60.11) - Unpurified [NB500-205] - HeLa cells stained with Survivin Antibody (60.11) - Unpurified [NB500-205] (Green) and detected with DyLight Fluor 488 conjugated anti-mouse IgG secondary antibody. Nuclei are counterstained with Hoechst 33258 (Blue).
Immunohistochemistry-Paraffin: Survivin Antibody (60.11) - Unpurified [NB500-205]

Immunohistochemistry-Paraffin: Survivin Antibody (60.11) - Unpurified [NB500-205]

Immunohistochemistry-Paraffin: Survivin Antibody (60.11) - Unpurified [NB500-205] - Immunohistochemical staining of formalin-fixed paraffin-embedded ovarian cancer tissue using Survivin Antibody (60.11) - Unpurified [NB500-205].
View 2 more images

Applications for Survivin Antibody (60.11) - Unpurified

Application
Recommended Usage

Flow Cytometry

1:200

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry-Frozen

reported in scientific literature (PMID 22841823)

Immunohistochemistry-Paraffin

reported in scientific literature (PMID 18332262)

Immunoprecipitation

1:10-1:500

Proximity Ligation Assay

reported in scientific literature (PMID 28077791)

Western Blot

reported in scientific literature (PMID 18332262)
Application Notes
This antibody weakly detects Survivin in Western blot; for this application we suggest using NB500-235 instead. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Ascites with PBS

Format

Unpurified

Preservative

0.05% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Survivin

Survivin (BIRC5 or IAP4) is the smallest member of the inhibitor of apoptosis (IAP) proteins, containing only a single baculovirus IAP repeat (BIR) domain and lacking the C-terminal RING domain. Survivin has a theoretical molecular weight of 16.5 kDa (wild-type protein) and exists as ten splice variant forms including the most predominant isoforms, survivin-2B and surviving-DeltaEx3 (1). It also undergoes phosphorylation by PKA, Plk1, Cdk1, CKII and Aurora B at multiple sites (e.g. Serine 20 and Threonine 34, 53 and 117).

Besides being highly abundant in fetal development and expressed in proliferating adult cells such as activated T lymphocytes, erythroblasts, and self-renewing stem cells, survivin is generally absent in adult tissues. However, it is elevated in common cancers such as lung, colon, pancreas, breast and prostate where it drives proliferation, metastasis, poor prognosis, and decreased patient survival (2).

Survivin has been shown to be involved in multiple cellular processes including cell cycle progression, mitotic spindle assembly, kinetochore attachment, angiogenesis, migration, and its anti-apoptotic activity has been linked to both its monomeric and homodimeric forms. Survivin impacts the function of other IAP members, c-IAP1 and c-IAP-2, or modulates the inhibitory activity of XIAP against caspases by forming a stable complex with XIAP and HBXIP. During the intrinsic apoptotic pathway, survivin may prevent the release of mitochondrial APAF1 into the cytoplasm or hinder the association of SMAC with other IAPS, which results in prolonged cell survival (3).

References

1. Sah NK, Seniya C. (2015) Survivin splice variants and their diagnostic significance. Tumour Biol. 36(9):6623-31. PMID: 26245993

2. Lladser A, Sanhueza C, Kiessling R, Quest AF. (2011) Is survivin the potential Achilles' heel of cancer? Adv Cancer Res. 111:1-37. PMID: 21704829

3. Wheatley SP, Altieri DC. (2019) Survivin at a glance. J Cell Sci. 132(7). PMID: 30948431

Alternate Names

API4, BIRC5

Entrez Gene IDs

332 (Human); 11799 (Mouse); 64041 (Rat)

Gene Symbol

BIRC5

UniProt

O15392

Additional Survivin Products

Product Documents for Survivin Antibody (60.11) - Unpurified

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Survivin Antibody (60.11) - Unpurified

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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