Survivin Antibody (8E2)

Novus Biologicals, part of Bio-Techne | Catalog # NB500-644

Novus Biologicals, part of Bio-Techne
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NB500-644
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Rat (98%). Backed by our 100% Guarantee.

Applications

Validated:

Block/Neutralize, ELISA, Flow (Intracellular), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, In vitro assay

Cited:

Block/Neutralize, ELISA, Flow Cytometry, IF/IHC, Immunoblotting, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 8E2

Concentration

1 mg/ml

View all Survivin Antibodies »

Product Specifications

Immunogen

This Survivin Antibody (8E2) was developed against full length recombinant human Survivin [UniProt# O15392]

Localization

Cytoplasm. Nucleus. Chromosome. Chromosome > centromere. Cytoplasm > cytoskeleton >spindle. Note: Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalizes with AURKB at mitotic chromosomes.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

16 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Survivin Antibody (8E2)

Immunohistochemistry: Survivin Antibody (8E2) [NB500-644]

Immunohistochemistry: Survivin Antibody (8E2) [NB500-644]

Immunohistochemistry: Survivin Antibody (8E2) [NB500-644] - Immunohistochemical analysis of Survivin in human breast cancer tissue using Survivin Antibody (8E2) [NB500-644] and DAB with hematoxylin counterstain.
Immunocytochemistry/ Immunofluorescence: Survivin Antibody (8E2) [NB500-644]

Immunocytochemistry/ Immunofluorescence: Survivin Antibody (8E2) [NB500-644]

Immunocytochemistry/Immunofluorescence: Survivin Antibody (8E2) [NB500-644] - Immunocytochemical analysis using Survivin Antibody (8E2) [NB500-644] was tested in HeLa cells with FITC (green). Nuclei were counterstained with DAPI (blue).
Flow (Intracellular): Survivin Antibody (8E2) [NB500-644]

Flow (Intracellular): Survivin Antibody (8E2) [NB500-644]

Flow (Intracellular): Survivin Antibody (8E2) [NB500-644] - An intracellular stain was performed on Daudi cells with Survivin Antibody (8E2) [NB500-644] (blue) and a matched isotype control NBP2-27287 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0101B, R&D Systems).

Applications for Survivin Antibody (8E2)

Application
Recommended Usage

Block/Neutralize

reported in scientific literature (PMID 14614132)

ELISA

reported in scientific literature (PMID 9581817)

Flow Cytometry

1 ug/mL. Use reported in scientific literature (PMID 11313271)

Immunocytochemistry/ Immunofluorescence

1:50-1:100

Immunohistochemistry

1:100

Immunohistochemistry-Paraffin

1:100
Application Notes
For IHC, heat induced antigen retreval using citrate buffer is required.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

Tris-Glycine and 0.15M NaCl

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Survivin

Survivin (BIRC5 or IAP4) is the smallest member of the inhibitor of apoptosis (IAP) proteins, containing only a single baculovirus IAP repeat (BIR) domain and lacking the C-terminal RING domain. Survivin has a theoretical molecular weight of 16.5 kDa (wild-type protein) and exists as ten splice variant forms including the most predominant isoforms, survivin-2B and surviving-DeltaEx3 (1). It also undergoes phosphorylation by PKA, Plk1, Cdk1, CKII and Aurora B at multiple sites (e.g. Serine 20 and Threonine 34, 53 and 117).

Besides being highly abundant in fetal development and expressed in proliferating adult cells such as activated T lymphocytes, erythroblasts, and self-renewing stem cells, survivin is generally absent in adult tissues. However, it is elevated in common cancers such as lung, colon, pancreas, breast and prostate where it drives proliferation, metastasis, poor prognosis, and decreased patient survival (2).

Survivin has been shown to be involved in multiple cellular processes including cell cycle progression, mitotic spindle assembly, kinetochore attachment, angiogenesis, migration, and its anti-apoptotic activity has been linked to both its monomeric and homodimeric forms. Survivin impacts the function of other IAP members, c-IAP1 and c-IAP-2, or modulates the inhibitory activity of XIAP against caspases by forming a stable complex with XIAP and HBXIP. During the intrinsic apoptotic pathway, survivin may prevent the release of mitochondrial APAF1 into the cytoplasm or hinder the association of SMAC with other IAPS, which results in prolonged cell survival (3).

References

1. Sah NK, Seniya C. (2015) Survivin splice variants and their diagnostic significance. Tumour Biol. 36(9):6623-31. PMID: 26245993

2. Lladser A, Sanhueza C, Kiessling R, Quest AF. (2011) Is survivin the potential Achilles' heel of cancer? Adv Cancer Res. 111:1-37. PMID: 21704829

3. Wheatley SP, Altieri DC. (2019) Survivin at a glance. J Cell Sci. 132(7). PMID: 30948431

Alternate Names

API4, BIRC5

Entrez Gene IDs

332 (Human); 64041 (Rat)

Gene Symbol

BIRC5

UniProt

O15392, Q9JHY7 (Rat)

Additional Survivin Products

Product Documents for Survivin Antibody (8E2)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Survivin Antibody (8E2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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