SYK Antibody (SYK-01)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-03250

Novus Biologicals, part of Bio-Techne
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NBP1-03250
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Knockout Validated, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # SYK-01

Concentration

0.5 mg/ml

View all SYK Antibodies »

Product Specifications

Immunogen

Recombinant human SYK.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for SYK Antibody (SYK-01)

Knockout Validated: SYK Antibody (SYK-01) [NBP1-03250]

Knockout Validated: SYK Antibody (SYK-01) [NBP1-03250]

Knockout Validated: SYK Antibody (SYK-01) [NBP1-03250] - Lysates of THP-1 (WT and SYK KO) were prepared and 30 ug of protein were processed for immunoblot with SYK Antibody (SYK-01). The Ponceau stained transfer is shown. Antibody dilution used: 1/500. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).
Western Blot: SYK Antibody (SYK-01) [NBP1-03250]

Western Blot: SYK Antibody (SYK-01) [NBP1-03250]

Western Blot: SYK Antibody (SYK-01) [NBP1-03250] - Western blot of extracts from Human PBMC, SYK Antibody (SYK-01)
Immunohistochemistry-Paraffin: SYK Antibody (SYK-01) [NBP1-03250]

Immunohistochemistry-Paraffin: SYK Antibody (SYK-01) [NBP1-03250]

Immunohistochemistry-Paraffin: SYK Antibody (SYK-01) [NBP1-03250] - Staining of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

Applications for SYK Antibody (SYK-01)

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

10 ug/ml

Immunoprecipitation

1:10-1:500

Western Blot

1:500
Application Notes
IHC: NBP1-03250 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity..

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2)

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: SYK

Syk (72 kDa) is a non-receptor protein tyrosine kinase that plays an important role in immune receptor signal transduction and is implicated in endothelial cell functions, including cell growth and migration. SYK is a positive effector of BCR stimulated responses. It couples the B cell antigen receptor (BCR) to the mobilization of calcium ions either through a phosphoinositide 3 kinase dependent pathway, when not phosphorylated on tyrosines of the linker region, or through a phospholipase C gamma dependent pathway, when phosphorylated on Tyr 342 and Tyr 346. Thus the differential phosphorylation of SYK can determine the pathway by which BCR is coupled to the regulation of intracellular calcium ions.

Long Name

Spleen Tyrosine Kinase

Alternate Names

DKFZp313N1010, EC 2.7.10, EC 2.7.10.2, FLJ25043, spleen tyrosine kinaseFLJ37489, tyrosine-protein kinase SYK

Entrez Gene IDs

20963 (Mouse); 25155 (Rat)

Gene Symbol

SYK

UniProt

P43405

Additional SYK Products

Product Documents for SYK Antibody (SYK-01)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for SYK Antibody (SYK-01)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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