Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Immunoprecipitation, Western Blot (Negative)

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all Symplekin Antibodies »

Product Summary for Symplekin Antibody

Immunogen

The immunogen recognized by this antibody maps to a region between residue 200 and 250 of human Symplekin using the numbering given in entry NP_004810.2 (GeneID 8189).

Localization

Cytoplasm, cytoskeleton. Cell junction, adherens junction; Cytoplasmic side. Cell junction; Cytoplasmic side. Nucleus, nucleoplasm. Note=Cytoplasmic face of adhesion plaques (major) and nucleoplasm (minor) (in cells with TJ). Nucleoplasm (in cells without

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Symplekin Antibody

Immunohistochemistry: Symplekin Antibody [NB100-74590]

Immunohistochemistry: Symplekin Antibody [NB100-74590]

Immunohistochemistry: Symplekin Antibody [NB100-74590] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- Symplekin used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Immunoprecipitation: Symplekin Antibody [NB100-74590]

Immunoprecipitation: Symplekin Antibody [NB100-74590]

Immunoprecipitation: Symplekin Antibody [NB100-74590] - Detection of human Symplekin by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Symplekin antibody NB100-74590 (lot 2) used for IP at 6 ug per reaction. Symplekin was also immunoprecipitated by a previous lot of this antibody (lot 1). For blotting immunoprecipitated Symplekin, NB100-74590 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Applications for Symplekin Antibody

Application
Recommended Usage

Immunoprecipitation

1:10-1:500
Application Notes
This antibody is not recommended for Western Blot analysis.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Symplekin

This gene encodes a nuclear protein that functions in the regulation of polyadenylation and promotes gene expression. The protein forms a high-molecular weight complex with components of the polyadenylation machinery. It is thought to serve as a scaffold for recruiting regulatory factors to the polyadenylation complex. It also participates in 3'-end maturation of histone mRNAs, which do not undergo polyadenylation. The protein also localizes to the cytoplasmic plaques of tight junctions in some cell types.

Alternate Names

FLJ27092, SPKSYM, symplekin

Entrez Gene IDs

8189 (Human)

Gene Symbol

SYMPK

UniProt

NP_004810.2 (Human)

Additional Symplekin Products

Product Documents for Symplekin Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Symplekin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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