Synaptophysin Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-25170

Novus Biologicals, part of Bio-Techne
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NBP2-25170SS
NBP2-25170
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Primate

Cited:

Human, Mouse, Rat

Predicted:

Bovine (96%), Rat (95%), Squirrel (97%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Cited:

Flow Cytometry, IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all Synaptophysin Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to an C-terminal portion of the human Synaptophysin protein (between residues 230-313) [UniProt# P08247]

Localization

Golgi, cytoplasmic and membrane

Marker

Pre-synaptic marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Synaptophysin Antibody - BSA Free

Western Blot: Synaptophysin AntibodyBSA Free [NBP2-25170]

Western Blot: Synaptophysin AntibodyBSA Free [NBP2-25170]

Western Blot: Synaptophysin Antibody [NBP2-25170] - Analysis of Synaptophysin in (A) human brain lysate and (B) mouse brain lysate.
Immunocytochemistry/ Immunofluorescence: Synaptophysin Antibody - BSA Free [NBP2-25170]

Immunocytochemistry/ Immunofluorescence: Synaptophysin Antibody - BSA Free [NBP2-25170]

Immunocytochemistry/Immunofluorescence: Synaptophysin Antibody [NBP2-25170] - PC12 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-Synaptophysin at a 1:50 dilution overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse DyLight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry: Synaptophysin Antibody - BSA Free [NBP2-25170]

Immunohistochemistry: Synaptophysin Antibody - BSA Free [NBP2-25170]

Immunohistochemistry: Synaptophysin Antibody [NBP2-25170] - Analysis of Synaptophysin in mouse brain.

Applications for Synaptophysin Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:250

Immunohistochemistry

1:100

Immunohistochemistry-Frozen

reported in scientific literature (PMID 31117227)

Immunohistochemistry-Paraffin

1:100

Western Blot

1.0 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Synaptophysin

Communication in the nervous system is dependent on the exocytosis of neurotransmitter-containing synaptic vesicles that release their contents at the synapses following membrane depolarization. Synaptophysin (SYP) is a major 38 kDa tetraspan membrane protein of small synaptic vesicles that plays an essential role in targeting synaptic vesicles to the presynaptic membrane. Synaptophysin directly interacts with the essential membrane fusion v-SNARE protein, synaptobrevin/VAMP2, and targets it to the presynaptic membrane (PMID: 14528015). Thus, synaptophysin is required for proper targeting of VAMP2 and synaptic vesicles. Synaptophysin is localized to the trans-Golgi cisternae and then accumulates to the presynaptic membrane in a microtubule dependent manner (PMID: 8227215).

Alternate Names

SYP

Entrez Gene IDs

6855 (Human); 20977 (Mouse)

Gene Symbol

SYP

UniProt

P08247, Q62277 (Mouse)

Additional Synaptophysin Products

Product Documents for Synaptophysin Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Synaptophysin Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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