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Synaptotagmin 1 Antibody (HL1626) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25705

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-25705

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse, Rat, Drosophila

Predicted:

Bovine (100%), Chicken (96%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL1626

Format

Azide and BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant fragment of human Synaptotagmin 1

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Synaptotagmin 1 Antibody (HL1626) - Azide and BSA Free

Synaptotagmin 1 Antibody (HL1626) - Azide and BSA Free

Western Blot: Synaptotagmin 1 Antibody (HL1626) - Azide and BSA Free [NBP3-25705] -

Western Blot: Synaptotagmin 1 Antibody (HL1626) - Azide and BSA Free [NBP3-25705] - Drosophila tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Synaptotagmin 1 antibody [HL1626] (NBP3-25705) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Synaptotagmin 1 Antibody (HL1626) - Azide and BSA Free

Western Blot: Synaptotagmin 1 Antibody (HL1626) - Azide and BSA Free [NBP3-25705] -

Western Blot: Synaptotagmin 1 Antibody (HL1626) - Azide and BSA Free [NBP3-25705] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Synaptotagmin 1 antibody [HL1626] (NBP3-25705) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Synaptotagmin 1 Antibody (HL1626) - Azide and BSA Free

Western Blot: Synaptotagmin 1 Antibody (HL1626) - Azide and BSA Free [NBP3-25705] -

Western Blot: Synaptotagmin 1 Antibody (HL1626) - Azide and BSA Free [NBP3-25705] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Synaptotagmin 1 antibody [HL1626] (NBP3-25705) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Applications for Synaptotagmin 1 Antibody (HL1626) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Synaptotagmin-1

Synaptotagmin is widely regarded as the primary calcium sensor for synaptic vesicle exocytosis (Fernandez-Chacon et al., 2001; Wang et al., 2003). Moreover recent studies indicate that the protein also plays a key role in endocytosis (Poskanzer et al., 2003). Synaptotagmin can be phosphorylated by multiple protein kinases and this may play a key role in modulation of synaptotagmin ability to influence both the exocytotic and endocytotic components of synaptic transmission (Hilfiker et al., 1999; Lee et al., 2004).

Alternate Names

P65, SVP65, Synaptotagmin1, SYT1

Gene Symbol

SYT1

Additional Synaptotagmin-1 Products

Product Documents for Synaptotagmin 1 Antibody (HL1626) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Synaptotagmin 1 Antibody (HL1626) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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