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Synaptotagmin 2 Antibody (1G10)

Novus Biologicals, part of Bio-Techne | Catalog # H00127833-M01

Novus Biologicals, part of Bio-Techne
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H00127833-M01

Key Product Details

Species Reactivity

Human

Applications

ELISA, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1G10

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

SYT2 (NP_796376, 311 a.a. ~ 418 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. KIHLMQNGKRLKKKKTTVKKKTLNPYFNESFSFEIPFEQIQKVQVVVTVLDYDKLGKNEAIGKIFVGSNATGTELRHWSDMLANPRRPIAQWHSLKPEEEVDALLGKN

Reactivity Notes

Other species not tested.

Localization

Cytoplasmic vesicle, secretory vesicle, synaptic vesicle. Chromaffin granule.

Specificity

SYT2 - synaptotagmin II

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for Synaptotagmin 2 Antibody (1G10)

Western Blot: Synaptotagmin 2 Antibody (1G10) [H00127833-M01]

Western Blot: Synaptotagmin 2 Antibody (1G10) [H00127833-M01]

Western Blot: Synaptotagmin 2 Antibody (1G10) [H00127833-M01] - SYT2 monoclonal antibody (M01), clone 1G10. Analysis of SYT2 expression in human kidney.
Western Blot: Synaptotagmin 2 Antibody (1G10) [H00127833-M01]

Western Blot: Synaptotagmin 2 Antibody (1G10) [H00127833-M01]

Western Blot: Synaptotagmin 2 Antibody (1G10) [H00127833-M01] - SYT2 monoclonal antibody (M01), clone 1G10 Western Blot analysis of SYT2 expression in HepG2 ( Cat # L019V1 ).
Sandwich ELISA: Synaptotagmin 2 Antibody (1G10) [H00127833-M01] - Detection limit for recombinant GST tagged SYT2 is approximately 0.1ng/ml as a capture antibody.

Applications for Synaptotagmin 2 Antibody (1G10)

Application
Recommended Usage

ELISA

1:100-1:2000

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for western blot. It has also been used for ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Synaptotagmin 2

SYT2 (Synaptotagmin II) belongs to the synaptotagmin family of multi-domained, integral membrane proteins of synaptic vesicles which are thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone.

Alternate Names

FLJ42519, synaptotagmin 2, synaptotagmin IISytII, synaptotagmin-2

Entrez Gene IDs

127833 (Human)

Gene Symbol

SYT2

OMIM

600104 (Human)

Additional Synaptotagmin 2 Products

Product Documents for Synaptotagmin 2 Antibody (1G10)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Synaptotagmin 2 Antibody (1G10)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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