Syndecan-1/CD138 Antibody (1A3H4) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-37282

Novus Biologicals, part of Bio-Techne
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NBP2-37282SS
NBP2-37282
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

CyTOF-ready, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Simple Western

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 1A3H4

Format

BSA Free

Concentration

1 mg/ml

View all Syndecan-1/CD138 Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human Syndecan-1/CD138 (AA: 28-171) expressed in E. Coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

32.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Syndecan-1/CD138 Antibody (1A3H4) - BSA Free

Western Blot: Syndecan-1/CD138 Antibody (1A3H4)BSA Free [NBP2-37282]

Western Blot: Syndecan-1/CD138 Antibody (1A3H4)BSA Free [NBP2-37282]

Western Blot: Syndecan-1/CD138 Antibody (1A3H4) [NBP2-37282] - Analysis using SDC1 mouse mAb against MCF-7 (1), Hela (2), HepG2 (3), T47D (4), SW620 (5), Jurkat (6) and NIH/3T3 (7) cell lysate.
Immunocytochemistry/ Immunofluorescence: Syndecan-1/CD138 Antibody (1A3H4) - BSA Free [NBP2-37282]

Immunocytochemistry/ Immunofluorescence: Syndecan-1/CD138 Antibody (1A3H4) - BSA Free [NBP2-37282]

Immunocytochemistry/Immunofluorescence: Syndecan-1/CD138 Antibody (1A3H4) [NBP2-37282] - Analysis of HepG2 cells using SDC1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.
Immunohistochemistry-Paraffin: Syndecan-1/CD138 Antibody (1A3H4) - BSA Free [NBP2-37282]

Immunohistochemistry-Paraffin: Syndecan-1/CD138 Antibody (1A3H4) - BSA Free [NBP2-37282]

Immunohistochemistry-Paraffin: Syndecan-1/CD138 Antibody (1A3H4) [NBP2-37282] - Analysis of rectum cancer tissues using SDC1 mouse mAb with DAB staining.
View 5 more images

Applications for Syndecan-1/CD138 Antibody (1A3H4) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Immunocytochemistry/ Immunofluorescence

1:200 - 1:1000

Immunohistochemistry

1:200 - 1:1000

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1:500 - 1:2000
Application Notes
This antibody is Cytof ready.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Syndecan-1/CD138

CD138, also known as Syndecan-1, is a transmembrane heparin sulfate proteoglycan that binds type I collagen. The relative molecular mass of CD138 is found to vary between 20-95 kDa due to the presence of a variable number of GAG modifications on each CD138 molecule. This protein appears to mediate binding of cells to the extracellular matrix. Among mature tissues, CD138 is expressed on simple and stratified epithelia, endothelial cells, and fibroblasts. (2,3) While stratified keratinocytes express CD138, their malignant transformation suppresses CD138 expression. Wound repair processes alter patterns of CD138 expression. Among hematopoietic cells, CD138 is expressed by immunoglobulin-negative pre-B cells in the bone marrow, immature B cells and by terminally differentiated plasma cells in lymphoid organs. CD138 expression has not been observed on mature, circulating peripheral B lymphocytes, however. (4-7) CD138 is also strongly expressed by multiple myeloma-derived cell lines and elevated levels of CD138 are present in the serum of patients with multiple myeloma, a negative prognostic indicator for the disease. (8)

Alternate Names

CD138, SDC1, Syndecan1

Gene Symbol

SDC1

Additional Syndecan-1/CD138 Products

Product Documents for Syndecan-1/CD138 Antibody (1A3H4) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Syndecan-1/CD138 Antibody (1A3H4) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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