Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # V92-P3D9*E7

Format

BSA Free

Concentration

1 mg/ml

View all Syndecan-1/CD138 Antibodies »

Product Specifications

Immunogen

Peptide Sequence - PAGPSQADLHTP

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for Syndecan-1/CD138 Antibody (V92-P3D9*E7) - BSA Free

Western Blot: Syndecan-1/CD138 Antibody (V92-P3D9/E7) [NBP2-50314]

Applications for Syndecan-1/CD138 Antibody (V92-P3D9*E7) - BSA Free

Application
Recommended Usage

ELISA

1:100 - 1:2000

Immunohistochemistry

1:10 - 1:500

Western Blot

1:100 - 1:2000
Application Notes
Positive control(s): ELISA - peptide immunogen; IHC - formalin-fixed, paraffin-embedded multi tumour tissue microarray; WB - Hela whole cell lysate. Observed product is slightly larger than anticipated due to multiple glycosylation sites.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Syndecan-1/CD138

CD138, also known as Syndecan-1, is a transmembrane heparin sulfate proteoglycan that binds type I collagen. The relative molecular mass of CD138 is found to vary between 20-95 kDa due to the presence of a variable number of GAG modifications on each CD138 molecule. This protein appears to mediate binding of cells to the extracellular matrix. Among mature tissues, CD138 is expressed on simple and stratified epithelia, endothelial cells, and fibroblasts. (2,3) While stratified keratinocytes express CD138, their malignant transformation suppresses CD138 expression. Wound repair processes alter patterns of CD138 expression. Among hematopoietic cells, CD138 is expressed by immunoglobulin-negative pre-B cells in the bone marrow, immature B cells and by terminally differentiated plasma cells in lymphoid organs. CD138 expression has not been observed on mature, circulating peripheral B lymphocytes, however. (4-7) CD138 is also strongly expressed by multiple myeloma-derived cell lines and elevated levels of CD138 are present in the serum of patients with multiple myeloma, a negative prognostic indicator for the disease. (8)

Alternate Names

CD138, SDC1, Syndecan1

Gene Symbol

SDC1

Additional Syndecan-1/CD138 Products

Product Documents for Syndecan-1/CD138 Antibody (V92-P3D9*E7) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Syndecan-1/CD138 Antibody (V92-P3D9*E7) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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