Syndecan-1/CD138 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-88135

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NBP1-88135
NBP1-88135-25ul
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Syndecan-1/CD138 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: TTTATTAQEPATSHPHRDMQPGHHETSTPAGPSQADLHTPHTEDGGPSATERAAEDGASSQLPAAEGSGEQDFTFETSGENTAVVAVEPDRRNQSPVDQ

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Syndecan-1/CD138 Antibody

Immunocytochemistry/ Immunofluorescence: Syndecan-1/CD138 Antibody [NBP1-88135]

Immunocytochemistry/ Immunofluorescence: Syndecan-1/CD138 Antibody [NBP1-88135]

Immunocytochemistry/Immunofluorescence: Syndecan-1/CD138 Antibody [NBP1-88135] - Staining of human cell line U-251 MG shows localization to focal adhesion sites. Antibody staining is shown in green.
Immunohistochemistry-Frozen: Syndecan-1/CD138 Antibody [NBP1-88135]

Immunohistochemistry-Frozen: Syndecan-1/CD138 Antibody [NBP1-88135]

Immunohistochemistry-Frozen: Syndecan-1/CD138 Antibody [NBP1-88135] - Human skin section stained with anti-CD138 (green). Sections were fixed, permeabilized, and blocked with 5% BSA solution. Antibody diluted 1:100 in block, applied for 1hr at 37C. Alexa Fluor 488-conjugated secondary was used, and counterstained with DAPI. Image submitted by a verified customer review.
Immunohistochemistry-Paraffin: Syndecan-1/CD138 Antibody [NBP1-88135]

Immunohistochemistry-Paraffin: Syndecan-1/CD138 Antibody [NBP1-88135]

Immunohistochemistry-Paraffin: Syndecan-1/CD138 Antibody [NBP1-88135] - Staining of human colon shows moderate to strong membranous positivity in lymphoid cells as well as in glandular cells.
View 3 more images

Applications for Syndecan-1/CD138 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization, Use PFA/Triton X-100. Syndecan-1/CD138 antibody validated for IHC-FR from a verified customer review.

Reviewed Applications

Read 1 review rated 4 using NBP1-88135 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Syndecan-1/CD138

CD138, also known as Syndecan-1, is a transmembrane heparin sulfate proteoglycan that binds type I collagen. The relative molecular mass of CD138 is found to vary between 20-95 kDa due to the presence of a variable number of GAG modifications on each CD138 molecule. This protein appears to mediate binding of cells to the extracellular matrix. Among mature tissues, CD138 is expressed on simple and stratified epithelia, endothelial cells, and fibroblasts. (2,3) While stratified keratinocytes express CD138, their malignant transformation suppresses CD138 expression. Wound repair processes alter patterns of CD138 expression. Among hematopoietic cells, CD138 is expressed by immunoglobulin-negative pre-B cells in the bone marrow, immature B cells and by terminally differentiated plasma cells in lymphoid organs. CD138 expression has not been observed on mature, circulating peripheral B lymphocytes, however. (4-7) CD138 is also strongly expressed by multiple myeloma-derived cell lines and elevated levels of CD138 are present in the serum of patients with multiple myeloma, a negative prognostic indicator for the disease. (8)

Alternate Names

CD138, SDC1, Syndecan1

Gene Symbol

SDC1

Additional Syndecan-1/CD138 Products

Product Documents for Syndecan-1/CD138 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Syndecan-1/CD138 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.