Syndecan-4 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-90173
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Summary for Syndecan-4 Antibody
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: PQDLLEGRYFSGALPDDEDVVGPGQESDDFELSGSGDLDDLEDSMIGPEVVHPLVPLDNHIPERAGSGSQVPTEPKKLEENEVIPKRISPVEESEDVSNKVSMSSTVQGSNIFERTEV
Marker
Plasma Membrane Marker
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Syndecan-4 Antibody
![Western Blot: Syndecan-4 Antibody [NBP1-90173]](https://resources.bio-techne.com/images/products/Syndecan-4-Antibody-Western-Blot-NBP1-90173-img0006.jpg "Western Blot: Syndecan-4 Antibody [NBP1-90173]")
Western Blot: Syndecan-4 Antibody [NBP1-90173]
Western Blot: Syndecan-4 Antibody [NBP1-90173] - Analysis in control (vector only transfected HEK293T lysate) and SDC4 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).![Immunocytochemistry/ Immunofluorescence: Syndecan-4 Antibody [NBP1-90173]](https://resources.bio-techne.com/images/products/Syndecan-4-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-90173-img0004.jpg "Immunocytochemistry/ Immunofluorescence: Syndecan-4 Antibody [NBP1-90173]")
Immunocytochemistry/ Immunofluorescence: Syndecan-4 Antibody [NBP1-90173]
Immunocytochemistry/Immunofluorescence: Syndecan-4 Antibody [NBP1-90173] - Staining of human cell line U-251 MG shows localization to plasma membrane & the Golgi apparatus. Antibody staining is shown in green.![Immunohistochemistry-Paraffin: Syndecan-4 Antibody [NBP1-90173]](https://resources.bio-techne.com/images/products/Syndecan-4-Antibody-Immunohistochemistry-Paraffin-NBP1-90173-img0005.jpg "Immunohistochemistry-Paraffin: Syndecan-4 Antibody [NBP1-90173]")
Immunohistochemistry-Paraffin: Syndecan-4 Antibody [NBP1-90173]
Immunohistochemistry-Paraffin: Syndecan-4 Antibody [NBP1-90173] - Staining of human duodenum shows strong cytoplasmic positivity in glandular cells.Applications for Syndecan-4 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:50 - 1:200
Immunohistochemistry-Paraffin
1:50 - 1:200
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Syndecan-4
Alternate Names
Amphiglycan, Ryudocan, SDC4, Syndecan4
Gene Symbol
SDC4
Additional Syndecan-4 Products
Product Documents for Syndecan-4 Antibody
Product Specific Notices for Syndecan-4 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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