Syntaxin 5 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-80771
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Bovine
Predicted:
Mouse (95%), Rat (95%). Backed by our 100% Guarantee.
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: QIAQLQDFVRAKGSQSGRHLQTHSNTIVVSLQSKLASMSNDFKSVLEVRTENLKQQRSRREQFSRAPVSALPLAPNHLGGGAVVLGAESHASKDVAIDMMDSRTSQQLQLIDEQDSYIQSRADTMQNIESTIVELGSIFQQLAHMV
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Syntaxin 5 Antibody
![Western Blot: Syntaxin 5 Antibody [NBP1-80771]](https://resources.bio-techne.com/images/products/Syntaxin-5-Antibody-Western-Blot-NBP1-80771-img0008.jpg "Western Blot: Syntaxin 5 Antibody [NBP1-80771]")
Western Blot: Syntaxin 5 Antibody [NBP1-80771]
Western Blot: Syntaxin 5 Antibody [NBP1-80771] - Analysis in control (vector only transfected HEK293T lysate) and STX5 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).![Immunocytochemistry/ Immunofluorescence: Syntaxin 5 Antibody [NBP1-80771]](https://resources.bio-techne.com/images/products/Syntaxin-5-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-80771-img0007.jpg "Immunocytochemistry/ Immunofluorescence: Syntaxin 5 Antibody [NBP1-80771]")
Immunocytochemistry/ Immunofluorescence: Syntaxin 5 Antibody [NBP1-80771]
Immunocytochemistry/Immunofluorescence: Syntaxin 5 Antibody [NBP1-80771] - Staining of human cell line U-2 OS shows localization to nucleoplasm & the Golgi apparatus. Antibody staining is shown in green.![Immunohistochemistry-Paraffin: Syntaxin 5 Antibody [NBP1-80771]](https://resources.bio-techne.com/images/products/Syntaxin-5-Antibody-Immunohistochemistry-Paraffin-NBP1-80771-img0001.jpg "Immunohistochemistry-Paraffin: Syntaxin 5 Antibody [NBP1-80771]")
Immunohistochemistry-Paraffin: Syntaxin 5 Antibody [NBP1-80771]
Immunohistochemistry-Paraffin: Syntaxin 5 Antibody [NBP1-80771] - Staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.Applications for Syntaxin 5 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:20 - 1:50
Immunohistochemistry-Paraffin
1:20 -1:50
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization, Use PFA/Triton X-100. Immunofluorescence verified by a customer review.
Reviewed Applications
Read 1 review rated 3 using NBP1-80771 in the following applications:
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Syntaxin 5
Additional Syntaxin 5 Products
Product Documents for Syntaxin 5 Antibody
Product Specific Notices for Syntaxin 5 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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