t-Plasminogen Activator/tPA Antibody (10W4L1)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16356
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 10W4L1
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for t-Plasminogen Activator/tPA Antibody (10W4L1)
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 463-562 of human tPA/t-Plasminogen Activator/tPA (P00750). LKEAHVRLYPSSRCTSQHLLNRTVTDNMLCAGDTRSGGPQANLHDACQGDSGGPLVCLNDGRMTLVGIISWGLGCGQKDVPGVYTKVTNYLDWIRDNMRP
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for t-Plasminogen Activator/tPA Antibody (10W4L1)
![Western Blot: t-Plasminogen Activator/tPA Antibody (10W4L1) [NBP3-16356]](https://resources.bio-techne.com/images/products/t-Plasminogen-Activator-tPA-Antibody-10W4L1-Western-Blot-NBP3-16356-img0002.jpg "Western Blot: t-Plasminogen Activator/tPA Antibody (10W4L1) [NBP3-16356]")
Western Blot: t-Plasminogen Activator/tPA Antibody (10W4L1) [NBP3-16356]
Western Blot: t-Plasminogen Activator/tPA Antibody (10W4L1) [NBP3-16356] - Western blot analysis of extracts of various cell lines, using tPA/t-Plasminogen Activator/tPA Rabbit mAb (NBP3-16356) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Immunocytochemistry/Immunofluorescence: t-Plasminogen Activator/tPA Antibody (10W4L1) [NBP3-16356] -
Immunocytochemistry/Immunofluorescence: t-Plasminogen Activator/tPA Antibody (10W4L1) [NBP3-16356] - Human placenta using tPA/PLAT Rabbit mAb (at dilution of 1:100) (Red). DAPI was used for nuclear staining (blue). Objective: 40x. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IF staining protocol.
Immunocytochemistry/Immunofluorescence: t-Plasminogen Activator/tPA Antibody (10W4L1) [NBP3-16356] -
Immunocytochemistry/Immunofluorescence: t-Plasminogen Activator/tPA Antibody (10W4L1) [NBP3-16356] - NIH/3T3 cells using tPA/PLAT Rabbit mAb (at dilution of 1:100) (Red). The cells were counterstained with alpha-Tubulin Mouse mAb (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.Applications for t-Plasminogen Activator/tPA Antibody (10W4L1)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Western Blot
1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: t-Plasminogen Activator/tPA
Long Name
Tissue Plasminogen Activator
Alternate Names
Alteplase, PLAT, Reteplase, T-PA, tPlasminogen Activator
Gene Symbol
PLAT
Additional t-Plasminogen Activator/tPA Products
Product Documents for t-Plasminogen Activator/tPA Antibody (10W4L1)
Product Specific Notices for t-Plasminogen Activator/tPA Antibody (10W4L1)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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