T Plastin Antibody (GT3310)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13518

Novus Biologicals, part of Bio-Techne
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NBP3-13518
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (95%), Chicken (95%), Rhesus Macaque (100%), Xenopus (91%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # GT3310

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all T Plastin Antibodies »

Product Summary for T Plastin Antibody (GT3310)

Immunogen

Recombinant protein encompassing a sequence within the center region of human T Plastin. The exact sequence is proprietary.

Reactivity Notes

Zebrafish (89%)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Description

Centrifuge briefly prior to opening.

Scientific Data Images for T Plastin Antibody (GT3310)

Western Blot: T Plastin Antibody (GT3310) [NBP3-13518]

Western Blot: T Plastin Antibody (GT3310) [NBP3-13518]

Western Blot: T Plastin Antibody (GT3310) [NBP3-13518] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with T Plastin antibody [GT3310] (NBP3-13518) diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: T Plastin Antibody (GT3310) [NBP3-13518]

Immunocytochemistry/ Immunofluorescence: T Plastin Antibody (GT3310) [NBP3-13518]

Immunocytochemistry/Immunofluorescence: T Plastin Antibody (GT3310) [NBP3-13518] - T Plastin antibody [GT3310] detects T Plastin protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: T Plastin protein stained by T Plastin antibody [GT3310] (NBP3-13518) diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 um.
Immunohistochemistry-Paraffin: T Plastin Antibody (GT3310) [NBP3-13518]

Immunohistochemistry-Paraffin: T Plastin Antibody (GT3310) [NBP3-13518]

Immunohistochemistry-Paraffin: T Plastin Antibody (GT3310) [NBP3-13518] - T Plastin antibody [GT3310] detects T Plastin protein at cytoplasm in rat liver by immunohistochemical analysis. Sample: Paraffin-embedded rat liver. T Plastin antibody [GT3310] (NBP3-13518) diluted at 1:250. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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Applications for T Plastin Antibody (GT3310)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: T Plastin

Plastins are a family of actin-binding proteins that are conserved throughout eukaryote evolution and expressed in most tissues of higher eukaryotes. In humans, two ubiquitous plastin isoforms (L and T) have been identified. Plastin 1 (otherwise known as Fimbrin) is a third distinct plastin isoform which is specifically expressed at high levels in the small intestine. The L isoform is expressed only in hemopoietic cell lineages, while the T isoform has been found in all other normal cells of solid tissues that have replicative potential (fibroblasts, endothelial cells, epithelial cells, melanocytes, etc.). The C-terminal 570 amino acids of the T-plastin and L-plastin proteins are 83% identical. It contains a potential calcium-binding site near the N terminus. Two transcript variants encoding the same protein have been found for this gene. (provided by RefSeq)

Alternate Names

plastin 3, plastin 3 (T isoform), plastin-3, T plastin, T-plastinT fimbrin

Gene Symbol

PLS3

Additional T Plastin Products

Product Documents for T Plastin Antibody (GT3310)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for T Plastin Antibody (GT3310)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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