T Plastin Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-06608

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all T Plastin Antibodies »

Product Summary for T Plastin Antibody

Immunogen

Produced in rabbits immunized with purified, recombinant Human T Plastin (Accession#: P13797; Gly102-Asn375)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for T Plastin Antibody

Western Blot: T Plastin Antibody [NBP3-06608]

Western Blot: T Plastin Antibody [NBP3-06608]

Western Blot: T Plastin Antibody [NBP3-06608] - Lane A: Raji Whole Cell Lysate Lane B: K562 Whole Cell Lysate Lane C: HT1080 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique.Performed under reducing conditions. Predicted band size:70 kDa Observed band size:70 kDa
Immunohistochemistry-Paraffin: T Plastin Antibody [NBP3-06608]

Immunohistochemistry-Paraffin: T Plastin Antibody [NBP3-06608]

Immunohistochemistry-Paraffin: T Plastin Antibody [NBP3-06608] - Staining of human PLS3 in human kidney with rabbit polyclonal antibody at 1:300 dilution.
Immunoprecipitation: T Plastin Antibody [NBP3-06608]

Immunoprecipitation: T Plastin Antibody [NBP3-06608]

Immunoprecipitation: T Plastin Antibody [NBP3-06608] - Lane A:0.5 mg K562 Whole Cell Lysate 4 uL anti-PLS3 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-PLS3 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 60 kDa Observed band size :60 kDa

Applications for T Plastin Antibody

Application
Recommended Usage

ELISA

1:5000-1:10000

Immunohistochemistry-Paraffin

1:200-1:1000

Immunoprecipitation

1-5uL/mg of lysate

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: T Plastin

Plastins are a family of actin-binding proteins that are conserved throughout eukaryote evolution and expressed in most tissues of higher eukaryotes. In humans, two ubiquitous plastin isoforms (L and T) have been identified. Plastin 1 (otherwise known as Fimbrin) is a third distinct plastin isoform which is specifically expressed at high levels in the small intestine. The L isoform is expressed only in hemopoietic cell lineages, while the T isoform has been found in all other normal cells of solid tissues that have replicative potential (fibroblasts, endothelial cells, epithelial cells, melanocytes, etc.). The C-terminal 570 amino acids of the T-plastin and L-plastin proteins are 83% identical. It contains a potential calcium-binding site near the N terminus. Two transcript variants encoding the same protein have been found for this gene. (provided by RefSeq)

Alternate Names

plastin 3, plastin 3 (T isoform), plastin-3, T plastin, T-plastinT fimbrin

Gene Symbol

PLS3

Additional T Plastin Products

Product Documents for T Plastin Antibody

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Certificate of Analysis

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Product Specific Notices for T Plastin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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