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TACE/ADAM17 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-86546

Novus Biologicals, part of Bio-Techne
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NBP1-86546
NBP1-86546-25ul

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: SEYTVKWQDFFTGHVVGEPDSRVLAHIRDDDVIIRINTDGAEYNIEPLWRFVNDTKDKRMLVYKSEDIKNVSRLQSPKVCGYLKVDNEELLPKGLVDREPPEELVHRVKRRADPDPMKNTCKLLVVADHRFYR

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (85%), Rat (86%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TACE/ADAM17 Antibody

Immunocytochemistry/ Immunofluorescence: TACE/ADAM17 Antibody [NBP1-86546]

Immunocytochemistry/ Immunofluorescence: TACE/ADAM17 Antibody [NBP1-86546]

Immunocytochemistry/Immunofluorescence: TACE/ADAM17 Antibody [NBP1-86546] - Staining of human cell line U-2 OS shows positivity in cytoplasm. Antibody staining is shown in green.
TACE/ADAM17 Antibody

Immunohistochemistry-Paraffin: TACE/ADAM17 Antibody [NBP1-86546] -

Immunohistochemistry-Paraffin: TACE/ADAM17 Antibody [NBP1-86546] - Staining of human heart muscle shows moderate cytoplasmic positivity in cardiomyocytes.
TACE/ADAM17 Antibody

Immunohistochemistry-Paraffin: TACE/ADAM17 Antibody [NBP1-86546] -

Immunohistochemistry-Paraffin: TACE/ADAM17 Antibody [NBP1-86546] - Staining of human placenta shows strong membranous and cytoplasmic positivity in trophoblastic cells.

Applications for TACE/ADAM17 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TACE/ADAM17

ADAM17 encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biologic processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The protein encoded by this gene functions as a tumor necrosis factor-alpha converting enzyme; binds mitotic arrest deficient 2 protein; and also plays a prominent role in the activation of the Notch signaling pathway.

Long Name

TNF-alpha Converting Enzyme

Alternate Names

ADAM17, CD156b

Entrez Gene IDs

6868 (Human)

Gene Symbol

ADAM17

UniProt

Additional TACE/ADAM17 Products

Product Documents for TACE/ADAM17 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for TACE/ADAM17 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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