TAF12 Antibody (1E10)

Novus Biologicals, part of Bio-Techne | Catalog # H00006883-M11

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1E10

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all TAF12 Antibodies »

Product Specifications

Immunogen

TAF12 (AAH11986, 1 a.a. ~ 161 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MNQFGPSALINLSNFSSIKPEPASTPPQGSMANSTAVVKIPGTPGAGGRLSPENNQVLTKKKLQDLVREVDPNEQLDEDVEEMLLQIADDFIESVVTAACQLARHRKSSTLEVKDVQLHLERQWNMWIPGFGSEEIRPYKKACTTEAHKQRMALIRKTTKK

Specificity

TAF12 - TAF12 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 20kDa (1E10)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for TAF12 Antibody (1E10)

Western Blot: TAF12 Antibody (1E10) [H00006883-M11]

Western Blot: TAF12 Antibody (1E10) [H00006883-M11]

Western Blot: TAF12 Antibody (1E10) [H00006883-M11] - Analysis of TAF12 expression in Hela S3 NE (Cat # L013V3).
Immunocytochemistry/ Immunofluorescence: TAF12 Antibody (1E10) [H00006883-M11]

Immunocytochemistry/ Immunofluorescence: TAF12 Antibody (1E10) [H00006883-M11]

Immunocytochemistry/Immunofluorescence: TAF12 Antibody (1E10) [H00006883-M11] - Analysis of monoclonal antibody to TAF12 on HeLa cell. Antibody concentration 10 ug/ml
Sandwich ELISA: TAF12 Antibody (1E10) [H00006883-M11] - Detection limit for recombinant GST tagged TAF12 is 0.3 ng/ml as a capture antibody.

Applications for TAF12 Antibody (1E10)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: TAF12

Control of transcription by RNA polymerase II involves the basal transcription machinery which is a collection of proteins. These proteins with RNA polymerase II, assemble into complexes which are modulated by transactivator proteins that bind to cis-regulatory elements located adjacent to the transcription start site. Some modulators interact directly with the basal complex, whereas others may act as bridging proteins linking transactivators to the basal transcription factors. Some of these associated factors are weakly attached while others are tightly associated with TBP in the TFIID complex. Among the latter are the TAF proteins. Different TAFs are predicted to mediate the function of distinct transcriptional activators for a variety of gene promoters and RNA polymerases. TAF12 interacts directly with TBP as well as with TAF2I. [provided by RefSeq]

Alternate Names

20kDa, TAF12 RNA polymerase II, TATA box binding protein (TBP)-associated factor, TAF15, TAF2J, TAFII-20/TAFII-15, TAFII20TAFII20/TAFII15, TATA box binding protein (TBP)-associated factor, RNA polymerase II, J, 20kD, Transcription initiation factor TFIID 20/15 kDa subunits, transcription initiation factor TFIID subunit 12

Entrez Gene IDs

6883 (Human)

Gene Symbol

TAF12

UniProt

AAH11986 (Human)

Additional TAF12 Products

Product Documents for TAF12 Antibody (1E10)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for TAF12 Antibody (1E10)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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