Key Product Details

Species Reactivity

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all TAF15 Antibodies »

Product Specifications

Immunogen

A synthetic peptide which maps to a region between residues 175 and 225 of human TAFII68 using the numbering given in Swiss-Prot entry Q92804 (GeneID 8148).

Specificity

NB100-566 is specific for human TAFII-68 protein.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TAF15 Antibody

Immunohistochemistry-Paraffin: TAF15 Antibody [NB100-566]

Immunohistochemistry-Paraffin: TAF15 Antibody [NB100-566]

Immunohistochemistry-Paraffin: TAF15 Antibody [NB100-566] - Sample: FFPE section of human testicular seminoma. Antibody: Affinity purified rabbit anti- TAFII68 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB
Immunoprecipitation: TAF15 Antibody [NB100-566]

Immunoprecipitation: TAF15 Antibody [NB100-566]

Immunoprecipitation: TAF15 Antibody [NB100-566] - Detection of human TAFII68 on HeLa whole cell lysate using NB100-566. TAFII68 was IPed with rabbit anti-TAFII68 antibodies NB100-566, NB100-567, NB100-568, and another using each at 0.3 ug/mg lysate.

Applications for TAF15 Antibody

Application
Recommended Usage

Immunohistochemistry

1:1000 - 1:5000

Immunohistochemistry-Paraffin

1:1000 - 1:5000

Immunoprecipitation

1-4 ug/mg lysate

Western Blot

1:5000-1:20000
Application Notes
Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use. In some cases, the antibody may be diluted further than indicated. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: TAF15

Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation.

Alternate Names

68kDa, hTAFII68, Npl3, RBP56TAFII68, RNA-binding protein 56, TAF(II)68, TAF15 RNA polymerase II, TATA box binding protein (TBP)-associated factor, TAF2NRBP56/CSMF fusion, TATA box binding protein (TBP)-associated factor, RNA polymerase II, N, 68kD(RNA-binding protein 56), TATA box-binding protein-associated factor 2N (RNA-binding protein 56), TATA-binding protein-associated factor 2N, TBP-associated factor 15,68 kDa TATA-binding protein-associated factor

Entrez Gene IDs

8148 (Human)

Gene Symbol

TAF15

UniProt

Q92804 (Human)

Additional TAF15 Products

Product Documents for TAF15 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for TAF15 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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