Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI2A5

Concentration

1 mg/ml

View all TAF2 Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 782-1121 of human TAF2(NP_003175) produced in E.coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

136.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for TAF2 Antibody (OTI2A5)

Western Blot: TAF2 Antibody (OTI2A5) [NBP2-01967]

Western Blot: TAF2 Antibody (OTI2A5) [NBP2-01967]

Western Blot: TAF2 Antibody (OTI2A5) [NBP2-01967] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TAF2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TAF2.

Applications for TAF2 Antibody (OTI2A5)

Application
Recommended Usage

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: TAF2

Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes one of the larger subunits of TFIID that is stably associated with the TFIID complex. It contributes to interactions at and downstream of the transcription initiation site, interactions that help determine transcription complex response to activators. [provided by RefSeq].

Alternate Names

150 kDa cofactor of initiator function, CIF150TAFII-150, cofactor of initiator function, 150kD subunit, TAF(II)150, TAF2 RNA polymerase II, TATA box binding protein (TBP)-associated factor, TAF2B, TAFII150RNA polymerase II TBP-associated factor subunit B, TATA box binding protein (TBP)-associated factor, RNA polymerase II, B, 150kD, TBP-associated factor 150 kDa, Transcription initiation factor TFIID 150 kDa subunit, transcription initiation factor TFIID subunit 2

Entrez Gene IDs

6873 (Human)

Gene Symbol

TAF2

Additional TAF2 Products

Product Documents for TAF2 Antibody (OTI2A5)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for TAF2 Antibody (OTI2A5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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