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Key Product Details

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot (Negative)

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1149 and 1199 of human TBP-associated factor 2 using the numbering given in entry NP_003175.1 (GeneID 6873).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TAF2 Antibody

Immunoprecipitation: TAF2 Antibody [NBP1-21372]

Immunoprecipitation: TAF2 Antibody [NBP1-21372]

Immunoprecipitation: TAF2 Antibody [NBP1-21372] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-TAF2 antibody used for IP at 10 mcg/mg lysate. TAF2 was also immunoprecipitated by rabbit anti-TAF2 antibody NBP1-21371, which recognizes a downstream epitope. For blotting immunoprecipitated TAF2, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Applications for TAF2 Antibody

Application
Recommended Usage

Immunoprecipitation

5-10 ug/mg lysate
Application Notes
It is not recommended for western analysis.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: TAF2

TAF2 is a component of the multi-subunit TFIID transcription factor. The TFIID complex is important for coordinating the pre-initiation complex and transcription by RNA Pol II. The complex contains the TFIID protein, TATA binding protein (TBP) and multiple TBP-associated factors (TAFs). TAF2 has been shown to play an important role in gene expression required for progression through the cell cycle.

Alternate Names

150 kDa cofactor of initiator function, CIF150TAFII-150, cofactor of initiator function, 150kD subunit, TAF(II)150, TAF2 RNA polymerase II, TATA box binding protein (TBP)-associated factor, TAF2B, TAFII150RNA polymerase II TBP-associated factor subunit B, TATA box binding protein (TBP)-associated factor, RNA polymerase II, B, 150kD, TBP-associated factor 150 kDa, Transcription initiation factor TFIID 150 kDa subunit, transcription initiation factor TFIID subunit 2

Entrez Gene IDs

6873 (Human)

Gene Symbol

TAF2

Additional TAF2 Products

Product Documents for TAF2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for TAF2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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