TAF6 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-60640

Novus Biologicals, part of Bio-Techne
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NB100-60640
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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

View all TAF6 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 627 and 677 of human TBP-associated factor 6 using the numbering given in entry NP_005632.1 (GeneID 6878).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TAF6 Antibody

Western Blot: TAF6 Antibody [NB100-60640]

Western Blot: TAF6 Antibody [NB100-60640]

Western Blot: TAF6 Antibody [NB100-60640] - Detection of Human TAF6 on HeLa whole cell lysate. TAF6 was also immunoprecipitated by rabbit anti-TAF6 antibody NB100-60639.
Immunohistochemistry-Paraffin: TAF6 Antibody [NB100-60640]

Immunohistochemistry-Paraffin: TAF6 Antibody [NB100-60640]

Immunohistochemistry-Paraffin: TAF6 Antibody [NB100-60640] - Section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- TAF6 used at a dilution of 1:200 (1ug/ml). Detection: DAB

Applications for TAF6 Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:500

Immunohistochemistry-Paraffin

1:100-1:500

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: TAF6

Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes one of the smaller subunits of TFIID that binds weakly to TBP but strongly to TAF1, the largest subunit of TFIID. Four isoforms have been identified but complete transcripts have been determined for only three isoforms. One of the isoforms has been shown to preclude binding of one of the other TFIID subunits, thereby reducing transcription and initiating signals that trigger apoptosis.

Alternate Names

DKFZp781E21155, MGC:8964, RNA polymerase II, E, 70/85kD, TAF(II)70, TAF(II)80, TAF2ERNA polymerase II TBP-associated factor subunit E, TAF6 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 80kDa, TAFII70TAFII-80, TAFII80TAFII-70, TAFII85, Transcription initiation factor TFIID 70 kDa subunit, Transcription initiation factor TFIID 80 kDa subunit, transcription initiation factor TFIID subunit 6

Entrez Gene IDs

6878 (Human)

Gene Symbol

TAF6

UniProt

NP_005632.1 (Human)

Additional TAF6 Products

Product Documents for TAF6 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for TAF6 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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