TAK1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-56363

Novus Biologicals, part of Bio-Techne
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NB100-56363SS
NB100-56363
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.5 mg/ml

View all TAK1 Antibodies »

Product Specifications

Immunogen

This polyclonal antibody was raised against amino acids 19-35 and 564-579 of human TAK1 (accession number BAA25025).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TAK1 Antibody

Western Blot: TAK1 Antibody [NB100-56363]

Western Blot: TAK1 Antibody [NB100-56363]

Western Blot: TAK1 Antibody [NB100-56363] - Western blot analysis of TAK1 in cell lysates from NIH 3T3 cells using NB100-56363 at 1 ug/ml dilution.
Immunocytochemistry/ Immunofluorescence: TAK1 Antibody [NB100-56363]

Immunocytochemistry/ Immunofluorescence: TAK1 Antibody [NB100-56363]

Immunocytochemistry/Immunofluorescence: TAK1 Antibody [NB100-56363] - RH30 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-TAK1 Antibody NB100-56363 at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.
Immunohistochemistry-Paraffin: TAK1 Antibody [NB100-56363]

Immunohistochemistry-Paraffin: TAK1 Antibody [NB100-56363]

Immunohistochemistry-Paraffin: TAK1 Antibody [NB100-56363] - Analysis of a FFPE tissue section of human stomach using 1:200 dilution of TAK1 antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.
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Applications for TAK1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-5 ug/ml

Immunohistochemistry

1:200. Use reported in scientific literature (PMID 30786091)

Immunohistochemistry-Paraffin

1:200

Western Blot

1-2 ug/ml
Application Notes
In NIH 3T3, an approx. 70 kDa band is observed.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS containing 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TAK1

TAK1 was shown to participate in regulation of transcription by transforming growth factor-beta (TGF-beta). TAK1 is stimulated in response to TGF-beta and bone morphogenetic protein. These results suggest that TAK1 functions as a mediator in the signaling pathway of TGF-beta superfamily members. TAB1 and TAB2 are TAK1 binding proteins that may function as activators of the TAK1 (TGF-b activated kinase 1) MAPKKK in TGF-b signal transduction. TAB1-induced TAK1 activation promoted the dissociation of active forms of IKKa and IKK b from active TAK1, whereas the IKK mutants remained to interact with active TAK1. TNF-a activated endogenous TAK1, and the kinase-negative TAK1 acted as a dominant negative inhibitor against TNF-a-induced NF-kB activation. TAK1 was suggested to act as a regulatory kinase of IKKs.

Long Name

TGF-beta activated kinase 1

Alternate Names

MAP3K7, TGF1a

Entrez Gene IDs

6885 (Human); 26409 (Mouse)

Gene Symbol

MAP3K7

UniProt

O43187

Additional TAK1 Products

Product Documents for TAK1 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for TAK1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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