Tau [p Thr231] Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-82249

Novus Biologicals, part of Bio-Techne
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NB100-82249
NB100-82249-0.025ml
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunohistochemistry-Frozen, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all Tau Antibodies »

Product Specifications

Immunogen

The antiserum was produced against synthesized phosphopeptide derived from human Tau around the phosphorylation site of threonine 231 (V-R-TP-P-P).

Modification

p Thr231

Specificity

Detects endogenous levels of Tau only when phosphorylated at threonine 231.

Marker

pan-Neuronal Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Tau [p Thr231] Antibody

Western Blot: Tau [p Thr231] Antibody [NB100-82249]

Western Blot: Tau [p Thr231] Antibody [NB100-82249]

Western Blot: Tau [p Thr231] Antibody [NB100-82249] - Western blot analysis of extracts from mouse brain tissue using NB100-82249 and the same antibody preincubated with blocking peptide.
Immunohistochemistry: Tau [p Thr231] Antibody [NB100-82249]

Immunohistochemistry: Tau [p Thr231] Antibody [NB100-82249]

Immunohistochemistry: Tau [p Thr231] Antibody [NB100-82249] - Immunohistochemical analysis of paraffin-embedded rat hippocampal region tissue from a model with Alzheimer's Disease using Tau (phospho-Thr231) antibody.

Applications for Tau [p Thr231] Antibody

Application
Recommended Usage

Immunohistochemistry

1:50-1:100

Immunohistochemistry-Paraffin

1:50-1:100

Western Blot

1:500-1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Tau

Tau is a neuronal microtubule associated protein found predominantly on axons. The function of Tau is to promote tubulin polymerisation and stabilise microtubules, but it also serves to link certain signalling pathways to the cytoskeleton. Tau, in its hyperphosphorylated form, is the major component of paired helical filaments (PHF) and neurofibrillary lesions in Alzheimer's disease (AD) brain. Hyperphosphorylation impairs the microtubule binding function of Tau, resulting in the destabilisation of microtubules in AD brains, ultimately leading to the degeneration of the affected neurons. Hyperphosphorylated tau is also found in a range of other central nervous system disorders. Numerous serine/threonine kinases, including GSK3 beta, PKA, Cdk5, and casein kinase II can phosphorylate Tau.

Long Name

Microtubule-Associated Protein Tau

Alternate Names

MAPT, MSTD, MTBT1, Neurofibrillary tangle protein, PPND

Entrez Gene IDs

4137 (Human); 17762 (Mouse); 29477 (Rat)

Gene Symbol

MAPT

UniProt

P10636

Additional Tau Products

Product Documents for Tau [p Thr231] Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Tau [p Thr231] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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