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Tau Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-11044

Novus Biologicals, part of Bio-Techne
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NBP3-11044-100ug

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Dot Blot, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit

Format

BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

Recombinant Human Tau AA297-391 (dGAE) Fibril (NP_005901.2)

Localization

Cytoplasm, Axolemma, Axolemma Plasma Membrane, Axon, Cell Body, Cell membrane, Cytoplasmic Ribonucleoprotein Granule, Cytoplasmic Side, Cytoskeleton, Cytosol, Dendrite, Growth cone, Microtubule, Microtubule Associated Complex, Neurofibrillary Tangle, Neuronal Cell Body, Nuclear Periphery, Nuclear Speck, Nucleus, Peripheral membrane protein, Plasma Membrane, Tubulin Complex

Specificity

Detects ~10.2 kDa. Detects other forms of Tau.

Clonality

Polyclonal

Host

Rabbit

Scientific Data Images for Tau Antibody - BSA Free

Western Blot: Tau Antibody [NBP3-11044]

Western Blot: Tau Antibody [NBP3-11044]

Western Blot: Tau Antibody [NBP3-11044] - Western blot analysis of Mouse, Rat Brain showing detection of Multiple bands due to different isoforms/aggregation states Tau protein using Rabbit Anti-Tau Polyclonal Antibody. Lane 1: MW Ladder. Lane 2: Mouse brain (15 ug). Lane 2: Rat brain (15 ug). Load: 15 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Rabbit Anti-Tau Polyclonal Antibody at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-rabbit IgG:HRP at 1:4000 for 1 hour at RT with shaking . Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Predicted/Observed Size: Multiple bands due to different isoforms/aggregation states. Other Band(s): 50 kDa, 65 kDa, 100 kDa.
Dot Blot: Tau Antibody [NBP3-11044] - Dot blot analysis using Rabbit Anti-Tau Polyclonal Antibody. Tissue: Recombinant Tau Monomers and Fibrils. Species: Human. Primary Antibody: Rabbit Anti-Tau Polyclonal Antibody at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-rabbit IgG:HRP at 1:4000 for 1 hour at RT with shaking.

Applications for Tau Antibody - BSA Free

Application
Recommended Usage

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4), 50% Glycerol

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: Tau

Tau is a microtubule binding protein that promotes microtubule assembly and stability. Tau is found to be the major component of the paired helical filaments (PHFs) found in the brains of patients with Alzheimer disease (AD) (1,2). Tau is hyperphosphorylated in PHFs, and specific phosphorylation sites have been implicated in the loss of Tau's association with the membrane cortex during AD disease state, including Ser 199/202, Thr 231, and Ser 393/404 (3). Glycogen synthase kinase-3, or GSK-3, phosphorylates Tau on Ser 396 (4).

Long Name

Microtubule-Associated Protein Tau

Alternate Names

MAPT, MSTD, MTBT1, Neurofibrillary tangle protein, PPND

Gene Symbol

MAPT

Additional Tau Products

Product Documents for Tau Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Tau Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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