Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI1H9

Concentration

0.72 mg/ml

View all TAZ/WWTR1 Antibodies »

Product Summary for TAZ/WWTR1 Antibody (OTI1H9)

Immunogen

Full length human recombinant protein of human WWTR1(NP_057287) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

43.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for TAZ/WWTR1 Antibody (OTI1H9)

Western Blot: TAZ/WWTR1 Antibody (OTI1H9) [NBP2-01209]

Western Blot: TAZ/WWTR1 Antibody (OTI1H9) [NBP2-01209]

Western Blot: TAZ/WWTR1 Antibody (1H9) [NBP2-01209] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TAZ (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TAZ.
Immunohistochemistry-Paraffin: TAZ/WWTR1 Antibody (OTI1H9) [NBP2-01209]

Immunohistochemistry-Paraffin: TAZ/WWTR1 Antibody (OTI1H9) [NBP2-01209]

Immunohistochemistry-Paraffin: TAZ/WWTR1 Antibody (1H9) [NBP2-01209] - Staining of paraffin-embedded Human tonsil using anti-TAZ mouse monoclonal antibody.
Immunohistochemistry-Paraffin: TAZ/WWTR1 Antibody (OTI1H9) [NBP2-01209]

Immunohistochemistry-Paraffin: TAZ/WWTR1 Antibody (OTI1H9) [NBP2-01209]

Immunohistochemistry-Paraffin: TAZ/WWTR1 Antibody (1H9) [NBP2-01209] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-TAZ mouse monoclonal antibody.
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Applications for TAZ/WWTR1 Antibody (OTI1H9)

Application
Recommended Usage

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.72 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: TAZ/WWTR1

TAZ is a YAP-related protein that contains a WW domain and transcriptional activation region. TAZ plays a role in mesenchymal stem cell differentiation. The antibody was designed in such a way that should generate a TAZ specific-reagent able to differentiate between YAP and TAZ proteins in cells and tissues.

Long Name

Transcriptional Coactivator with PDZ-binding Motif

Alternate Names

WWTR1

Entrez Gene IDs

25937 (Human)

Gene Symbol

WWTR1

Additional TAZ/WWTR1 Products

Product Documents for TAZ/WWTR1 Antibody (OTI1H9)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for TAZ/WWTR1 Antibody (OTI1H9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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