Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat

Applications

Validated:

Chromatin Immunoprecipitation, Chromatin Immunoprecipitation (ChIP), Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Proximity Ligation Assay, Simple Western, Western Blot

Cited:

Chemotaxis, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all TAZ/WWTR1 Antibodies »

Product Specifications

Immunogen

Synthetic peptides made to the human TAZ protein. These peptides were selected for the lack of homology to YAP protein. [UniProt# Q9GZV5]

Localization

Nuclear and cytoplasmic. Concentrates along specific portions of the plasma membrane, and accumulates in punctate nuclear bodies.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TAZ/WWTR1 Antibody - BSA Free

Western Blot: TAZ/WWTR1 AntibodyBSA Free [NB110-58359]

Western Blot: TAZ/WWTR1 AntibodyBSA Free [NB110-58359]

Western Blot: TAZ/WWTR1 Antibody [NB110-58359] - Detection of TAZ on HEK 293 cell lysate using NB110-58359.
Immunocytochemistry/ Immunofluorescence: TAZ/WWTR1 Antibody - BSA Free [NB110-58359]

Immunocytochemistry/ Immunofluorescence: TAZ/WWTR1 Antibody - BSA Free [NB110-58359]

Immunocytochemistry/Immunofluorescence: TAZ/WWTR1 Antibody [NB110-58359] - Detection of TAZ (Green) in HepG2 cells using NB110-58359. Nuclei (Blue) are counterstained with Hoecsht 33258.
Immunohistochemistry-Paraffin: TAZ/WWTR1 Antibody - BSA Free [NB110-58359]

Immunohistochemistry-Paraffin: TAZ/WWTR1 Antibody - BSA Free [NB110-58359]

Immunohistochemistry-Paraffin: TAZ/WWTR1 Antibody [NB110-58359] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human kidney using TAZ/WWTR1 antibody at 1:500 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Nuclear and cytoplasmic staining was observed. Staining was performed by Histowiz.
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Applications for TAZ/WWTR1 Antibody - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation

reported in scientific literature (PMID 25587023)

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry

1:500

Immunohistochemistry-Paraffin

1:500. Use reported in scientific literature (PMID 25587023)

Immunoprecipitation

1:100

Proximity Ligation Assay

reported in scientific literature (PMID 25587023)

Simple Western

1:100

Western Blot

1:1000-1:5000
Application Notes
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 3 using NB110-58359 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: TAZ/WWTR1

TAZ (transcriptional co-activator with PDZ-binding motif) was identified as a binding partner of 14-3-3 family of proteins which contains seven homologous proteins with ability to bind phosphorylated serine with certain sequence motifs, thereby involving in diverse cellular functions such as differentiation, cell cycle progression and apoptosis via their interaction with diverse intracellular phosphoproteins involved in signaling networks. TAZ is homologous to YAP and displays transcriptional co-activator function via interaction with PPXY-containing transcriptional factors through its WW domain. Several transcriptional factors such as Runx/PEBP2, AP2, C/EBP, c-Jun, Krox-20, Krox-24, MEF2B, NF-E2, Oct-4 and p73 containing proline-rich PPXY motif have been proposed as TAZ's interacting partners. TAZ is also a major target of the Hippo core kinase cascade which regulates organ size control as well as stem cell properties by governing cell proliferation and apoptosis processes. Hippo mediated phosphorylation of YAP and TAZ leads to their sequestration into the cytoplasm by interaction with 14-3-3 proteins and ubiquitination-dependent proteosomal degradation, thereby governing its distribution, protein levels and functionality. TAZ interacts primarily with transcriptional factors TEAD1-4 (TEADs) and activates expression of target genes such as CTGF, IGFBP3, ITGB2, Birc5/Survivin, Gli2, and Axl.

Long Name

Transcriptional Coactivator with PDZ-binding Motif

Alternate Names

WWTR1

Entrez Gene IDs

25937 (Human)

Gene Symbol

WWTR1

UniProt

Q9GZV5 (Human)

Additional TAZ/WWTR1 Products

Product Documents for TAZ/WWTR1 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for TAZ/WWTR1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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