TAZ/WWTR1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-85067

Novus Biologicals, part of Bio-Techne
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NBP1-85067
NBP1-85067-25ul
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Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Predicted:

Rat (96%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Simple Western, Western Blot

Cited:

IF/IHC, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all TAZ/WWTR1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: MNPKPSSWRKKILPESFFKEPDSGSHSRQSSTDSSGGHPGPRLAGGAQHVRSHSSPASLQLGTGAGAAGSPAQQHAHLRQQSYDVTDELPLPPGWEMTFTATGQRYFLNHIEKITTWQDPRKAMNQPLNHMNLHPAVSST

Reactivity Notes

Reactivity reported in scientific literature (PMID: 24658687)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TAZ/WWTR1 Antibody

Immunohistochemistry-Paraffin: TAZ/WWTR1 Antibody [NBP1-85067]

Immunohistochemistry-Paraffin: TAZ/WWTR1 Antibody [NBP1-85067]

Immunohistochemistry-Paraffin: TAZ/WWTR1 Antibody [NBP1-85067] - Staining in human placenta and skeletal muscle tissues . Corresponding WWTR1 RNA-seq data are presented for the same tissues.
Western Blot: TAZ/WWTR1 Antibody [NBP1-85067]

Western Blot: TAZ/WWTR1 Antibody [NBP1-85067]

Western Blot: TAZ/WWTR1 Antibody [NBP1-85067] - Analysis in EFO-21 cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading control: Anti-PPIB.
Immunocytochemistry/ Immunofluorescence: TAZ/WWTR1 Antibody [NBP1-85067]

Immunocytochemistry/ Immunofluorescence: TAZ/WWTR1 Antibody [NBP1-85067]

Immunocytochemistry/Immunofluorescence: TAZ/WWTR1 Antibody [NBP1-85067] - Staining of human cell line U-251 MG shows localization to nucleoplasm, nuclear bodies and cytosol. Antibody staining is shown in green.
View 8 more images

Applications for TAZ/WWTR1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Simple Western

1:30

Western Blot

0.04-0.4 ug/ml
Application Notes
IHC reported in scientific literature (PMID:22190458). IHC-Paraffin HIER pH6 retrieval is recommended. ICC/IF Fixation/Permeabilization: PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TAZ/WWTR1

TAZ (transcriptional co-activator with PDZ-binding motif) was identified as a binding partner of 14-3-3 family of proteins which contains seven homologous proteins with ability to bind phosphorylated serine with certain sequence motifs, thereby involving in diverse cellular functions such as differentiation, cell cycle progression and apoptosis via their interaction with diverse intracellular phosphoproteins involved in signaling networks. TAZ is homologous to YAP and displays transcriptional co-activator function via interaction with PPXY-containing transcriptional factors through its WW domain. Several transcriptional factors such as Runx/PEBP2, AP2, C/EBP, c-Jun, Krox-20, Krox-24, MEF2B, NF-E2, Oct-4 and p73 containing proline-rich PPXY motif have been proposed as TAZ's interacting partners and TAZ is a major target of Hippo core kinase cascade which regulates organ size control as well as stem cell properties by governing cell proliferation and apoptosis processes Hippo mediated phosphorylation of YAP and TAZ leads to their sequestration into cytoplasm by interaction with 14-3-3 proteins and ubiquitination-dependent proteosomal degradation, thereby governing its distribution, protein levels and functionality also. TAZ interact primarily with transcriptional factors TEAD1-4 (TEADs) and activate expression of target genes such as CTGF, IGFBP3, ITGB2, Birc5/Survivin, Gli2, and Axl.

Long Name

Transcriptional Coactivator with PDZ-binding Motif

Alternate Names

WWTR1

Entrez Gene IDs

25937 (Human)

Gene Symbol

WWTR1

UniProt

Q9GZV5 (Human)

Additional TAZ/WWTR1 Products

Product Documents for TAZ/WWTR1 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for TAZ/WWTR1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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