TBC1D4 Antibody (OTI5E6) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-74462
![Flow Cytometry: TBC1D4 Antibody (OTI5E6) - Azide and BSA Free [NBP2-74462]](https://resources.bio-techne.com/images/products/TBC1D4-Antibody-OTI5E6-Azide-and-BSA-Free-Flow-Cytometry-NBP2-74462-img0002.jpg "Flow Cytometry: TBC1D4 Antibody (OTI5E6) - Azide and BSA Free [NBP2-74462]")
![Flow Cytometry: TBC1D4 Antibody (OTI5E6) - Azide and BSA Free [NBP2-74462]](https://resources.bio-techne.com/images/products/TBC1D4-Antibody-OTI5E6-Azide-and-BSA-Free-Flow-Cytometry-NBP2-74462-img0003.jpg "Flow Cytometry: TBC1D4 Antibody (OTI5E6) - Azide and BSA Free [NBP2-74462]")
![Western Blot: TBC1D4 Antibody (OTI5E6)Azide and BSA Free [NBP2-74462]](https://resources.bio-techne.com/images/products/TBC1D4-Antibody-OTI5E6-Azide-and-BSA-Free-Western-Blot-NBP2-74462-img0001.jpg "Western Blot: TBC1D4 Antibody (OTI5E6)Azide and BSA Free [NBP2-74462]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # OTI5E6
Format
Azide and BSA Free
Concentration
LYOPH mg/ml
Product Summary for TBC1D4 Antibody (OTI5E6) - Azide and BSA Free
Immunogen
Full length human recombinant protein of human TBC1D4 (NP_0055647) produced in HEK293T cell.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Theoretical MW
146.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for TBC1D4 Antibody (OTI5E6) - Azide and BSA Free
Western Blot: TBC1D4 Antibody (OTI5E6)Azide and BSA Free [NBP2-74462]
Western Blot: TBC1D4 Antibody (OTI5E6) - Azide and BSA Free [NBP2-74462] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TBC1D4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TBC1D4.![Immunocytochemistry/ Immunofluorescence: TBC1D4 Antibody (OTI5E6) - Azide and BSA Free [NBP2-74462]](https://resources.bio-techne.com/images/products/TBC1D4-Antibody-OTI5E6-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-74462-img0005.jpg "Immunocytochemistry/ Immunofluorescence: TBC1D4 Antibody (OTI5E6) - Azide and BSA Free [NBP2-74462]")
Immunocytochemistry/ Immunofluorescence: TBC1D4 Antibody (OTI5E6) - Azide and BSA Free [NBP2-74462]
Immunocytochemistry/Immunofluorescence: TBC1D4 Antibody (OTI5E6) - Azide and BSA Free [NBP2-74462] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY TBC1D4.![Flow Cytometry: TBC1D4 Antibody (OTI5E6) - Azide and BSA Free [NBP2-74462]](https://resources.bio-techne.com/images/products/TBC1D4-Antibody-OTI5E6-Azide-and-BSA-Free-Flow-Cytometry-NBP2-74462-img0004.jpg "Flow Cytometry: TBC1D4 Antibody (OTI5E6) - Azide and BSA Free [NBP2-74462]")
Flow Cytometry: TBC1D4 Antibody (OTI5E6) - Azide and BSA Free [NBP2-74462]
Flow Cytometry: TBC1D4 Antibody (OTI5E6) - Azide and BSA Free [NBP2-74462] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-TBC1D4 antibody, and then analyzed by flow cytometry.Applications for TBC1D4 Antibody (OTI5E6) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
1:100
Immunocytochemistry/ Immunofluorescence
1:100
Western Blot
1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Reconstitution
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.
Formulation
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: TBC1D4
Alternate Names
Akt substrate of 160 kDa, AS160Acrg embryonic lethality minimal region ortholog, DKFZp779C0666, KIAA0603TBC (Tre-2, BUB2, CDC16) domain-containing protein, TBC1 domain family member 4, TBC1 domain family, member 4
Gene Symbol
TBC1D4
Additional TBC1D4 Products
Product Documents for TBC1D4 Antibody (OTI5E6) - Azide and BSA Free
Product Specific Notices for TBC1D4 Antibody (OTI5E6) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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