TBC1D4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-57094

Novus Biologicals, part of Bio-Techne
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NB100-57094
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Canine (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all TBC1D4 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-DDPEKIEERKKSK corresponding to internal region according to NP_055647.2.

Epitope

DDPEKIEERKKSK

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for TBC1D4 Antibody

Western Blot: TBC1D4 Antibody [NB100-57094]

Western Blot: TBC1D4 Antibody [NB100-57094]

Western Blot: TBC1D4 Antibody [NB100-57094] - (0.1ug/ml) staining of Daudi cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: TBC1D4 Antibody [NB100-57094]

Immunohistochemistry-Paraffin: TBC1D4 Antibody [NB100-57094]

Immunohistochemistry-Paraffin: TBC1D4 Antibody [NB100-57094] - (3.8ug/ml) staining of Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for TBC1D4 Antibody

Application
Recommended Usage

Immunohistochemistry

3 - 5 ug/ml

Immunohistochemistry-Paraffin

3 - 5 ug/ml

Peptide ELISA

Detection limit 1:128000

Western Blot

0.1 - 0.3 ug/ml
Application Notes
WB: Approx. 150 kDa band observed in lysates of Daudi and MOLT4 (calculated MW of 147 kDa band according to NP_055647.2). IHC-P: Human skeletal muscle shows striated pattern staining of majority of muscular fibres.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: TBC1D4

AS160 is a 160 kDa Akt substrate that plays a critical role in regulating insulin-stimulated exocytosis of glucose transporter GLUT4. AS160 contains a Rab GTPase-activating protein domain (GAP), suggesting its potential role in membrane trafficking. Insulin-stimulated phosphorylation of AS160 on threonine 642 is critical for mediating GLUT4 translocation in fat and muscle cells, via inactivation of Rab GAP function. Phosphorylation of threonine 642 is also important in mediating AS160 redistribution from low density microsomes to the cytosol in adipocytes.

Alternate Names

Akt substrate of 160 kDa, AS160Acrg embryonic lethality minimal region ortholog, DKFZp779C0666, KIAA0603TBC (Tre-2, BUB2, CDC16) domain-containing protein, TBC1 domain family member 4, TBC1 domain family, member 4

Entrez Gene IDs

9882 (Human)

Gene Symbol

TBC1D4

UniProt

NP_055647.2 (Human)

Additional TBC1D4 Products

Product Documents for TBC1D4 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for TBC1D4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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