TBCA Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-97322

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all TBCA Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with E. coli-derived Human TBCA fragment.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for TBCA Antibody

Immunocytochemistry/ Immunofluorescence: TBCA Antibody [NBP2-97322]

Immunocytochemistry/ Immunofluorescence: TBCA Antibody [NBP2-97322]

Immunocytochemistry/Immunofluorescence: TBCA Antibody [NBP2-97322] - Immunofluorescence staining of TBCA in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human TBCA polyclonal antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Nucleus and Cytoplasm.
Immunohistochemistry-Paraffin: TBCA Antibody [NBP2-97322]

Immunohistochemistry-Paraffin: TBCA Antibody [NBP2-97322]

Immunohistochemistry-Paraffin: TBCA Antibody [NBP2-97322] - Immunochemical staining of human TBCA in human skeletal muscle with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.
Immunohistochemistry-Paraffin: TBCA Antibody [NBP2-97322]

Immunohistochemistry-Paraffin: TBCA Antibody [NBP2-97322]

Immunohistochemistry-Paraffin: TBCA Antibody [NBP2-97322] - Immunochemical staining of human TBCA in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.

Applications for TBCA Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:500

Immunohistochemistry-Paraffin

1:50-1:200

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

PBS

Preservative

0.03% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TBCA

Tubulin folding cofactor A, also known as TBCA, is one of four proteins (cofactors A, D, E, and C) involved in the pathway leading to correctly folded beta-tubulin from folding intermediates. Cofactors A and D are believed to play a role in capturing and stabilizing beta-tubulin in a quasi-native confirmation. This protein is essential for cell viability and its knockdown produces a decrease in the amount of soluble tubulin, modifications in microtubules and G1 cell cycle arrest. Recombinant human TBCA protein was expressed in E.coli and purified by using conventional chromatography techniques.

Alternate Names

CFA, chaperonin cofactor A, TCP1-chaperonin cofactor A, tubulin folding cofactor A, Tubulin-folding cofactor A, tubulin-specific chaperone a

Gene Symbol

TBCA

Additional TBCA Products

Product Documents for TBCA Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for TBCA Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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