TBK1 Antibody (108A429) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-33243

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Bovine, Canine

Predicted:

Chimpanzee (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockout Validated, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 108A429

Format

Azide and BSA Free

Concentration

1.0 mg/ml

View all TBK1 Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to amino acids 563-577 (YYQFKKDKAERRLAYC) of human TBK1 was used as immunogen for this antibody (NP_037386).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

84 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for TBK1 Antibody (108A429) - Azide and BSA Free

Western Blot: TBK1 Antibody (108A429)Azide and BSA Free [NBP2-33243]

Western Blot: TBK1 Antibody (108A429)Azide and BSA Free [NBP2-33243]

Western Blot: TBK1 Antibody (108A429) - Azide and BSA Free [NBP2-33243] - Human Daudi (A) and mouse RAW (B) cell lysate probed with TBK1 antibody at 2 ug/ml.
Immunocytochemistry/ Immunofluorescence: TBK1 Antibody (108A429) - Azide and BSA Free [NBP2-33243]

Immunocytochemistry/ Immunofluorescence: TBK1 Antibody (108A429) - Azide and BSA Free [NBP2-33243]

Immunocytochemistry/Immunofluorescence: TBK1 Antibody (108A429) - Azide and BSA Free [NBP2-33243] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti-TBK1 at 20 ug/ml overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective. Image using the standard format of this product.
Immunohistochemistry-Paraffin: TBK1 Antibody (108A429) - Azide and BSA Free [NBP2-33243]

Immunohistochemistry-Paraffin: TBK1 Antibody (108A429) - Azide and BSA Free [NBP2-33243]

Immunohistochemistry-Paraffin: TBK1 Antibody (108A429) - Azide and BSA Free [NBP2-33243] - Analysis of FFPE human testis tissue section using Peripheral Node Addressin antibody (clone MECA-79R) at 1:200. The staining was developed with HRP-DAB detection method and the counterstaining was performed using hematoxylin. Image using the standard format of this product.
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Applications for TBK1 Antibody (108A429) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10 - 1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1-2 ug/ml
Application Notes
In Western Blot this antibody detects a band at 80 kDa.In Simple Western only 10-15 uL of the recommended dilution is used per data point.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS - BSA free

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TBK1

NF-kB (nuclear factor kB) is sequestered in the cytoplasm by IkB family of inhibitory proteins that mask the nuclear localization signal of NF-kB thereby preventing translocation of NF-kB to the nucleus. External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Synthesis of IkBa is autoregulated. IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g. IKK1/a and IKK2/b are phosphorylated by NF-kB-inducing kinase (NIK) and MAP kinase kinase kinase-1 (MEKK1), respectively. Recently, a new IKK family member, TBK1 [(TANK-binding kinase), also known as NAK and T2K] has been identified. TANK (TRAF-associated NF-kB activator) interacts with TBK1 and activates NF-kB in a kinase dependent manner.

Long Name

TANK Binding Kinase 1

Alternate Names

FTDALS4, NAK, NFKB-Activating Kinase

Entrez Gene IDs

29110 (Human); 56480 (Mouse); 299827 (Rat)

Gene Symbol

TBK1

UniProt

Q9UHD2

Additional TBK1 Products

Product Documents for TBK1 Antibody (108A429) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for TBK1 Antibody (108A429) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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