TBK1 Antibody (4G6C7)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16189

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 4G6C7

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all TBK1 Antibodies »

Product Summary for TBK1 Antibody (4G6C7)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human TBK1 (Q9UHD2). MQSTSNHLWLLSDILGQGATANVFRGRHKKTGDLFAIKVFNNISFLRPVDVQMREFEVLKKLNHKNIVKLFAIEEETTTRHKVLIMEFCPCGSLYTVLEE

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

84 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for TBK1 Antibody (4G6C7)

Western Blot: TBK1 Antibody (4G6C7) [NBP3-16189]

Western Blot: TBK1 Antibody (4G6C7) [NBP3-16189]

Western Blot: TBK1 Antibody (4G6C7) [NBP3-16189] - Western blot analysis of extracts of various cell lines, using TBK1 Rabbit mAb (NBP3-16189) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Immunocytochemistry/ Immunofluorescence: TBK1 Antibody (4G6C7) [NBP3-16189]

Immunocytochemistry/ Immunofluorescence: TBK1 Antibody (4G6C7) [NBP3-16189]

Immunocytochemistry/Immunofluorescence: TBK1 Antibody (4G6C7) [NBP3-16189] - Immunofluorescence analysis of U-2 OS cells using TBK1 Rabbit mAb (NBP3-16189) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Western Blot: TBK1 Antibody (4G6C7) [NBP3-16189]

Western Blot: TBK1 Antibody (4G6C7) [NBP3-16189]

Western Blot: TBK1 Antibody (4G6C7) [NBP3-16189] - Western blot analysis of extracts of various cell lines, using TBK1 Rabbit mAb (NBP3-16189) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
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Applications for TBK1 Antibody (4G6C7)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunoprecipitation

0.5μg-4μg antibody for 200μg-400μg extracts of whole cells

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: TBK1

NFkappaB-activating kinase (NAK) also known as TANK binding kinase-1 (TBK1) or tumor necrosis factor receptor associated factor 2-associated kinase (T2K) is a serine/threonine protein that belongs to the IkB kinase (IKK) family (1). Similar to IKK alpha and beta (2), NAK induces IkB degradation and NF-kB activity. NAK is activated by phorbol ester tumour promoters and growth factors, whereas catalytically inactive NAK specifically inhibits activation of NF-kB by protein kinase C-epsilon (PKCepsilon). NAK specifically phosphorylates IkB alpha on Serine 36 and NFkB subunit RelA (p65) on Serine 536 (3). NAK is also known to interact with TANK, TRAF-2 and TRIF (4).

Long Name

TANK Binding Kinase 1

Alternate Names

FTDALS4, NAK, NFKB-Activating Kinase

Gene Symbol

TBK1

Additional TBK1 Products

Product Documents for TBK1 Antibody (4G6C7)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for TBK1 Antibody (4G6C7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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