TBX5 Antibody (1G10)

Novus Biologicals, part of Bio-Techne | Catalog # H00006910-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Fish, Monkey

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1G10

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all TBX5 Antibodies »

Product Specifications

Immunogen

TBX5 (AAH27942, 402 a.a. ~ 518 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. PSMPSYSSCTVTTVQPMDRLPYQHFSAHFTSGPLVPRLAGMANHGSPQLGEGMFQHQTSVAHQPVVRQCGPQTGLQSPGTLQPPEFLYSHGVPRTLSPHQYHSVHGVGMVPEWSDNS

Specificity

TBX5 - T-box 5

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for TBX5 Antibody (1G10)

Western Blot: TBX5 Antibody (1G10) [H00006910-M01]

Western Blot: TBX5 Antibody (1G10) [H00006910-M01]

Western Blot: TBX5 Antibody (1G10) [H00006910-M01] - Analysis of TBX5 over-expressed 293 cell line, cotransfected with TBX5 Validated Chimera RNAi ( Cat # H00006910-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with TBX5 monoclonal antibody (M01), clone 1G10 (Cat # H00006910-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western Blot: TBX5 Antibody (1G10) [H00006910-M01]

Western Blot: TBX5 Antibody (1G10) [H00006910-M01]

Western Blot: TBX5 Antibody (1G10) [H00006910-M01] - Analysis of TBX5 expression in transfected 293T cell line by TBX5 monoclonal antibody (M01), clone 1G10.Lane 1: TBX5 transfected lysate(57.7 KDa).Lane 2: Non-transfected lysate.

Applications for TBX5 Antibody (1G10)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against recombinant protein for WB. It has been used for RNAi Validation and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: TBX5

This gene is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. This gene is closely linked to related family member T-box 3 (ulnar mammary syndrome) on human chromosome 12. The encoded protein may play a role in heart development and specification of limb identity. Mutations in this gene have been associated with Holt-Oram syndrome, a developmental disorder affecting the heart and upper limbs. Several transcript variants encoding different isoforms have been described for this gene.

Long Name

T-box Transcription Factor 5

Alternate Names

HOS, T-box 5, T-box protein 5, T-box transcription factor TBX5

Entrez Gene IDs

6910 (Human)

Gene Symbol

TBX5

OMIM

142 (Human)

UniProt

AAH27942 (Human)

Additional TBX5 Products

Product Documents for TBX5 Antibody (1G10)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for TBX5 Antibody (1G10)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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