TBX5 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-33715

Novus Biologicals, part of Bio-Techne
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NBP1-33715
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Key Product Details

Species Reactivity

Validated:

Human, Rat, Zebrafish

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all TBX5 Antibodies »

Product Summary for TBX5 Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human TBX5. The exact sequence is proprietary.

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

58 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for TBX5 Antibody

Western Blot: TBX5 Antibody [NBP1-33715]

Western Blot: TBX5 Antibody [NBP1-33715]

Western Blot: TBX5 Antibody [NBP1-33715] - HeLa whole cell and nuclear extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with TBX5 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: TBX5 Antibody [NBP1-33715]

Immunocytochemistry/ Immunofluorescence: TBX5 Antibody [NBP1-33715]

Immunocytochemistry/Immunofluorescence: TBX5 Antibody [NBP1-33715] - Paraformaldehyde-fixed HeLa, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: TBX5 Antibody [NBP1-33715]

Immunohistochemistry-Paraffin: TBX5 Antibody [NBP1-33715]

Immunohistochemistry-Paraffin: TBX5 Antibody [NBP1-33715] - Rat ovary. TBX5 antibody diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
View 3 more images

Applications for TBX5 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol, 1% BSA

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: TBX5

This gene is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. This gene is closely linked to related family member T-box 3 (ulnar mammary syndrome) on human chromosome 12. The encoded protein may play a role in heart development and specification of limb identity. Mutations in this gene have been associated with Holt-Oram syndrome, a developmental disorder affecting the heart and upper limbs. Several transcript variants encoding different isoforms have been described for this gene.

Long Name

T-box Transcription Factor 5

Alternate Names

HOS, T-box 5, T-box protein 5, T-box transcription factor TBX5

Gene Symbol

TBX5

Additional TBX5 Products

Product Documents for TBX5 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for TBX5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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