TcBuster-M Transposase Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB11511
Key Product Details
Species Reactivity
Multi-Species
Applications
Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 1077035
Product Specifications
Immunogen
Synthetic Peptide
Specificity
Detects TcBuster-M Transposase in Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for TcBuster-M Transposase Antibody
Detection of TcBuster-M Transposase by Western Blot.
Western Blot shows lysates of HEK293T human embryonic kidney cell line mock electroporated or electroporated with 1 µg TcBuster-M Transposase after 1 day post-electroporation. PVDF membrane was probed with 1 µg/ml of Mouse Anti-TcBuster-M Transposase Monoclonal Antibody (Catalog # MAB11511) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for TcBuster-M Transposase at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
Detection of TcBuster-M Transposase by Simple WesternTM.
Simple Western shows lysates HEK293T human embryonic kidney cell line mock electroporated or electroporated with 1µg TcBuster-M Transposase after 1 day post-electroporation, loaded at 0.5 mg/ml. A specific band was detected for TcBuster-M Transposase at approximately 71 kDa (as indicated) using 20 µg/mL of Mouse Anti-TcBuster-M Transposase Monoclonal Antibody (Catalog # MAB11511). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for TcBuster-M Transposase Antibody
Application
Recommended Usage
Simple Western
20 µg/mL
Sample: HEK293T human embryonic kidney cell line mock electroporated or electroporated with 1 µg TcBuster-M Transposase after 1 day post-electroporation
Sample: HEK293T human embryonic kidney cell line mock electroporated or electroporated with 1 µg TcBuster-M Transposase after 1 day post-electroporation
Western Blot
1 µg/mL
Sample: HEK293T human embryonic kidney cell line mock electroporated or electroporated with 1 µg TcBuster-M Transposase after 1 day post-electroporation
Sample: HEK293T human embryonic kidney cell line mock electroporated or electroporated with 1 µg TcBuster-M Transposase after 1 day post-electroporation
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TcBuster Transposase
References
- Woodard, L.E. et al. (2012) PLOS One DOI: 10.1371/journal.pone.0042666.
- Skeate, J.G. et al. (2024) Molecular Therapy DOI: 10.1016/j.ymthe.2024.04.024.
Long Name
Tribolium castaneumTcBuster Transposase Peptide
Alternate Names
GLEAN_06077, TcasGA2_TC006077, Transposase
Additional TcBuster Transposase Products
Product Documents for TcBuster-M Transposase Antibody
Product Specific Notices for TcBuster-M Transposase Antibody
For research use only
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