TCF-3/E2A Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-38611

Novus Biologicals, part of Bio-Techne
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NBP2-38611
NBP2-38611-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all TCF-3/E2A Antibodies »

Product Summary for TCF-3/E2A Antibody

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: RTFSEGTHFTESHSSLSSSTFLGPGLGGKSGERGAYASFGRDAGVGGLTQAGFLSGELALNSPGPLSPSGMKGTSQYYPS

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TCF-3/E2A Antibody

Immunocytochemistry/ Immunofluorescence: TCF-3/E2A Antibody [NBP2-38611]

Immunocytochemistry/ Immunofluorescence: TCF-3/E2A Antibody [NBP2-38611]

Immunocytochemistry/Immunofluorescence: TCF-3/E2A Antibody [NBP2-38611] - Staining of human cell line MCF7 shows localization to nuclear speckles. Antibody staning is shown in green.
Immunohistochemistry-Paraffin: TCF-3/E2A Antibody [NBP2-38611]

Immunohistochemistry-Paraffin: TCF-3/E2A Antibody [NBP2-38611]

Immunohistochemistry-Paraffin: TCF-3/E2A Antibody [NBP2-38611] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: TCF-3/E2A Antibody [NBP2-38611]

Immunohistochemistry-Paraffin: TCF-3/E2A Antibody [NBP2-38611]

Immunohistochemistry-Paraffin: TCF-3/E2A Antibody [NBP2-38611] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
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Applications for TCF-3/E2A Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TCF-3/E2A

TCF3 / E2A encodes a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific transcripts such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver. This gene has been linked to sporatic cases of maturity-onset diabetes of the young. Two transcript variants encoding the same protein have been identified for this gene.

Long Name

Transcription Factor 3/E2-alpha

Alternate Names

bHLHb21, E2A, ITF1, Kappa-E2-binding factor, Pan2, Tcfe2a, VDIR

Gene Symbol

TCF3

UniProt

P15923

Additional TCF-3/E2A Products

Product Documents for TCF-3/E2A Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for TCF-3/E2A Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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