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TCF21 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25175

Novus Biologicals, part of Bio-Techne
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NBP3-25175

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (96%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody has been engineered to specifically recognize the recombinant protein TCF21 using the following amino acid sequence: SSYIAHLRQILANDKYENGYIHPVNLTWPFMVAGKPESDLKEVVTASRLCGTTAS

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TCF21 Antibody

TCF21 Antibody Immunocytochemistry/Immunofluorescence: TCF21 Antibody [NBP3-25175]

Immunocytochemistry/Immunofluorescence: TCF21 Antibody [NBP3-25175]

Staining of human cell line A-549 shows localization to nucleoplasm.

Applications for TCF21 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 µg/ml
Application Notes
For ICC/IF, we recommend using a combination of PFA and Triton X-100. This will give you the optimal results for your experiments.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.2, 40% glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TCF21

The basic helix-loop-helix transcription factor TCF21/Pod1 (also called capsulin or epicardin) is involved in kidney, lung and spleen organogenesis. It is also essential for normal development of the testes and ovaries. TCF21/Pod1 is involved in the transcriptional repression of steroidogenic factor 1 (Sf1/Nr5a1/Ad4BP), an orphan nuclear receptor that regulates the expression of multiple genes (including Scc) that mediate sexual differentiation.

Alternate Names

bHLHa23BHLHA23, capsulin, Class A basic helix-loop-helix protein 23, Epicardin, Pod-1, POD1pod-1, Podocyte-expressed 1, TCF-21, transcription factor 21

Gene Symbol

TCF21

Additional TCF21 Products

Product Documents for TCF21 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for TCF21 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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