TCF7L2 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-19083

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NBP1-19083
NBP1-19083SS
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Bovine (93%), Mouse (93%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all TCF7L2 Antibodies »

Product Specifications

Immunogen

Synthetic peptide made to an internal portion of human TCF7L2 (within residues 150-200). [Swiss-Prot# Q9NQB0]

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: mouse (93%) and bovine (93%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for TCF7L2 Antibody - BSA Free

Western Blot: TCF7L2 AntibodyBSA Free [NBP1-19083]

Western Blot: TCF7L2 AntibodyBSA Free [NBP1-19083]

Western Blot: TCF7L2 Antibody [NBP1-19083] - Western blot analysis of TCF7L2 (F) protein in D3H2LN TNBC cells after treatment with SCR or SID decoy peptide (2.5uM, 24 hours). SID decoy treatment inhibited full-length (~64 kDa) and, to a greater extent, short isoform variant (~53 kDa) TCF7L2 proteins in comparison to untreated or SCR treated controls. The band intensities for TCF7L2 in each lane were normalized to the GAPDH loading control and indicated in red. The result is representative of three independent experiments. Image collected and cropped by CiteAb from the following publication (https://www.oncotarget.com/lookup/doi/10.18632/oncotarget.11381) licensed under a CC-BY license.
Immunocytochemistry/ Immunofluorescence: TCF7L2 Antibody - BSA Free [NBP1-19083]

Immunocytochemistry/ Immunofluorescence: TCF7L2 Antibody - BSA Free [NBP1-19083]

Immunocytochemistry/Immunofluorescence: TCF7L2 Antibody [NBP1-19083] - TCF7L2 antibody was tested in Hela cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
Western Blot: TCF7L2 AntibodyBSA Free [NBP1-19083]

Western Blot: TCF7L2 AntibodyBSA Free [NBP1-19083]

Western Blot: TCF7L2 Antibody [NBP1-19083] - Jurkat cell line.

Applications for TCF7L2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

0.5 ug/ml
Application Notes
This TCF7L2 antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where a band is seen ~55 kDa. In ICC/IF cytoplasmic staining was observed. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TCF7L2

TCF7L2 (transcription factor 7-like 2, also called TCF4) is a key component in WNT signal transduction pathway which is implicated in several fundamental cellular processes such as embryonic development, stem cell maintenance, cellular fate, proliferation, survival, migration, tumour suppression, and oncogenesis. TCF7L2 gene codes for HMG- box transcription factor and through its critical role in WNT/beta-catenin signaling, it plays important role in cellular development as well as growth regulation. TCF7L2 participates in the process of blood glucose homeostasis via its regulatory effects on GLP-1 (glucagon-like peptide 1) and gene variants rs12255372 as well as rs7903146 have been documented to be associated with risk for NIDDM/Type 2 diabetes. TCF7L2 also regulates the transcription of proglucagon gene in enteroendocrine cells and has been suggested to be implicated in obesity also. TCF7L2 has been shown associate with human carcinogenesis specifically with triple negative i.e. ER, PR, HER2 negative or basal-like breast cancers.

Alternate Names

HMG box transcription factor 4, hTCF-4, T-cell factor 4, T-cell factor-4 variant A, T-cell factor-4 variant B, T-cell factor-4 variant C, T-cell factor-4 variant D, T-cell factor-4 variant E, T-cell factor-4 variant H, T-cell factor-4 variant I, T-cell factor-4 variant J, T-cell factor-4 variant K, T-cell factor-4 variant L, T-cell factor-4 variant M, T-cell factor-4 variant X2, T-cell-specific transcription factor 4, TCF-4T-cell factor-4 variant F, TCF4T-cell factor-4 variant G, transcription factor 7-like 2, transcription factor 7-like 2 (T-cell specific, HMG-box)

Gene Symbol

TCF7L2

Additional TCF7L2 Products

Product Documents for TCF7L2 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for TCF7L2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.