TCF7L2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-12873

Novus Biologicals, part of Bio-Techne
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NBP3-12873
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (100%), Zebrafish (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all TCF7L2 Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human TCF7L2. The exact sequence is proprietary.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Centrifuge briefly prior to opening.

Scientific Data Images for TCF7L2 Antibody

Western Blot: TCF7L2 Antibody [NBP3-12873]

Western Blot: TCF7L2 Antibody [NBP3-12873]

Western Blot: TCF7L2 Antibody [NBP3-12873] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membranes were blotted with TCF7L2 antibody [N1N2], N-term (NBP3-12873) diluted at 1:500 and competitor's antibody diluted at 1:100. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: TCF7L2 Antibody [NBP3-12873]

Immunocytochemistry/ Immunofluorescence: TCF7L2 Antibody [NBP3-12873]

Immunocytochemistry/Immunofluorescence: TCF7L2 Antibody [NBP3-12873] - TCF7L2 antibody detects TCF7L2 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: TCF7L2 protein stained by TCF7L2 antibody (NBP3-12873) diluted at 1:500. Red: Phalloidin, a cytoskeleton marker, diluted at 1:100. Scale bar = 10 um.
Western Blot: TCF7L2 Antibody [NBP3-12873]

Western Blot: TCF7L2 Antibody [NBP3-12873]

Western Blot: TCF7L2 Antibody [NBP3-12873] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TCF7L2 antibody (NBP3-12873) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Applications for TCF7L2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TCF7L2

TCF7L2, or TCF4, is a high mobility group (HMG) box-containing transcription factor that plays a key role in the Wnt signaling pathway. TCF7L2 has been implicated in glucose homeostasis through the regulation of pro-glucagon gene expression, which encodes glucagon-like peptide 1 (GLP-1). Genetic variants of TCF7L2 have been linked to type 2 diabetes, and it has specifically been shown to have an important role in the regulation of both 946;-cell survival and function (4). Several splicing isoforms for TCF7L2 have been shown to be differentially expressed in tissues during cancer progression (3), and gene targeting studies indicate that TCF7L2 is required to maintain the crypt stem cells of the small intestine (1, 2).

Alternate Names

HMG box transcription factor 4, hTCF-4, T-cell factor 4, T-cell factor-4 variant A, T-cell factor-4 variant B, T-cell factor-4 variant C, T-cell factor-4 variant D, T-cell factor-4 variant E, T-cell factor-4 variant H, T-cell factor-4 variant I, T-cell factor-4 variant J, T-cell factor-4 variant K, T-cell factor-4 variant L, T-cell factor-4 variant M, T-cell factor-4 variant X2, T-cell-specific transcription factor 4, TCF-4T-cell factor-4 variant F, TCF4T-cell factor-4 variant G, transcription factor 7-like 2, transcription factor 7-like 2 (T-cell specific, HMG-box)

Gene Symbol

TCF7L2

Additional TCF7L2 Products

Product Documents for TCF7L2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for TCF7L2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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