TCP1-beta Antibody (9N6B4)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16465

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16465-100ul
NBP3-16465-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 9N6B4

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all TCP1-beta Antibodies »

Product Summary for TCP1-beta Antibody (9N6B4)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 436-535 of human TCP1-beta (P78371). MESYAKALRMLPTIIADNAGYDSADLVAQLRAAHSEGNTTAGLDMREGTIGDMAILGITESFQVKRQVLLSAAEAAEVILRVDNIIKAAPRKRVPDHHPC

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TCP1-beta Antibody (9N6B4)

Western Blot: TCP1-beta Antibody (9N6B4) [NBP3-16465]

Western Blot: TCP1-beta Antibody (9N6B4) [NBP3-16465]

Western Blot: TCP1-beta Antibody (9N6B4) [NBP3-16465] - Western blot analysis of extracts of various cell lines, using TCP1-beta Rabbit mAb (NBP3-16465) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
Immunohistochemistry-Paraffin: TCP1-beta Antibody (9N6B4) [NBP3-16465]

Immunohistochemistry-Paraffin: TCP1-beta Antibody (9N6B4) [NBP3-16465]

Immunohistochemistry-Paraffin: TCP1-beta Antibody (9N6B4) [NBP3-16465] - Immunohistochemistry of paraffin-embedded rat brain using TCP1-beta Rabbit mAb (NBP3-16465) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Applications for TCP1-beta Antibody (9N6B4)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: TCP1-beta

TCP1-beta encodes a molecular chaperone that is member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternate transcriptional splice variants of the gene described in this record have been observed but have not been thoroughly characterized.

Alternate Names

Cctb, CCT-beta, CCTBPRO1633,99D8.1, chaperonin containing t-complex polypeptide 1, beta subunit, chaperonin containing t-complex polypeptide 1, subunit 2, chaperonin containing TCP1, subunit 2 (beta), MGC142074, MGC142076, T-complex protein 1 subunit beta, T-complex protein 1, beta subunit, TCP-1-beta

Gene Symbol

CCT2

Additional TCP1-beta Products

Product Documents for TCP1-beta Antibody (9N6B4)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for TCP1-beta Antibody (9N6B4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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