TCP1-beta Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-88146

Novus Biologicals, part of Bio-Techne
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NBP1-88146
NBP1-88146-25ul
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Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation, Independent Antibodies

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all TCP1-beta Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: VNQPKRIENAKILIANTGMDTDKIKIFGSRVRVDSTAKVAEIEHAEKEKMKEKVERILKHGINCFINRQLIYNYPEQLFGAAGVMAIEHADFAGVERLALVTGGEIASTFDHPELVKLGSCKLIEEVMIGEDKLIHFSGV

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TCP1-beta Antibody

Immunohistochemistry-Paraffin: TCP1-beta Antibody [NBP1-88146]

Immunohistochemistry-Paraffin: TCP1-beta Antibody [NBP1-88146]

Immunohistochemistry-Paraffin: TCP1-beta Antibody [NBP1-88146] - Staining in human testis and skeletal muscle tissues using anti-CCT2 antibody. Corresponding CCT2 RNA-seq data are presented for the same tissues.
Western Blot: TCP1-beta Antibody [NBP1-88146]

Western Blot: TCP1-beta Antibody [NBP1-88146]

Western Blot: TCP1-beta Antibody [NBP1-88146] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2, using anti-CCT2 antibody. Remaining relative intensity is presented.
Immunohistochemistry-Paraffin: TCP1-beta Antibody [NBP1-88146]

Immunohistochemistry-Paraffin: TCP1-beta Antibody [NBP1-88146]

Immunohistochemistry-Paraffin: TCP1-beta Antibody [NBP1-88146] - Staining of human cerebral cortex, skeletal muscle, testis and tonsil using Anti-CCT2 antibody NBP1-88146 (A) shows similar protein distribution across tissues to independent antibody NBP1-88147 (B).
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Applications for TCP1-beta Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TCP1-beta

TCP1-beta encodes a molecular chaperone that is member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternate transcriptional splice variants of the gene described in this record have been observed but have not been thoroughly characterized.

Alternate Names

Cctb, CCT-beta, CCTBPRO1633,99D8.1, chaperonin containing t-complex polypeptide 1, beta subunit, chaperonin containing t-complex polypeptide 1, subunit 2, chaperonin containing TCP1, subunit 2 (beta), MGC142074, MGC142076, T-complex protein 1 subunit beta, T-complex protein 1, beta subunit, TCP-1-beta

Gene Symbol

CCT2

Additional TCP1-beta Products

Product Documents for TCP1-beta Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for TCP1-beta Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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