TCR beta Antibody (H57-597) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-20117
Recombinant Monoclonal Antibody
Conjugate
Catalog #
Key Product Details
Species Reactivity
Mouse
Applications
Flow Cytometry, Immunohistochemistry, Immunoprecipitation
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Armenian Hamster IgG Lambda Clone # H57-597
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Hamsters were immunised with affinity purified DO-11.10 TCR, and produced detectable anti-TCR antibodies which were judged by the ability of the anti-sera to immunoprecipitate TCR from surface labelled DO-11.10 T cell hybridomas. Hamster 57 was selected for hybridisation, and B cell hybridomas were produced using polyethylene glycol fusion of hamster splenic B cells to the mouse myeloma variant. Selected mAb were screened after subsequent culturing for their ability to immunoprecipitate TCR from detergent lysates of surface-radiolabelled murine T cell hybridomas.
Epitope
H57-597 is directed to an epitope within the FG loop of the C region of TCR beta chain.
Specificity
mAb H57-597 reacts with surface receptors on all alpha-beta TCR bearing cells, but does not react with receptors on gamma-delta T cells.
Clonality
Monoclonal
Host
Armenian Hamster
Isotype
IgG Lambda
Applications for TCR beta Antibody (H57-597) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunoprecipitation
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
In an immobilised form, this antibody can directly activate T cells bearing alpha-beta TCR to produce IL-2.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
0.02% Proclin 300
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
Background: TCR beta
Alternate Names
T cell receptor beta chain, TCRB, TCRbeta, Tib
Gene Symbol
Tcrb
Additional TCR beta Products
Product Documents for TCR beta Antibody (H57-597) - Azide and BSA Free
Product Specific Notices for TCR beta Antibody (H57-597) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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