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TCR beta Antibody (H57-597) - Chimeric - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-20118

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Lambda Clone # H57-597

Format

Azide and BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

Hamsters were immunised with affinity purified DO-11.10 TCR, and produced detectable anti-TCR antibodies which were judged by the ability of the anti-sera to immunoprecipitate TCR from surface labelled DO-11.10 T cell hybridomas. Hamster 57 was selected for hybridisation, and B cell hybridomas were produced using polyethylene glycol fusion of hamster splenic B cells to the mouse myeloma variant. Selected mAb were screened after subsequent culturing for their ability to immunoprecipitate TCR from detergent lysates of surface-radiolabelled murine T cell hybridomas.

Epitope

H57-597 is directed to an epitope within the FG loop of the C region of TCR beta chain.

Specificity

mAb H57-597 reacts with surface receptors on all alpha-beta TCR bearing cells, but does not react with receptors on gamma-delta T cells.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Lambda

Scientific Data Images for TCR beta Antibody (H57-597) - Chimeric - Azide and BSA Free

TCR beta Antibody (H57-597) - Chimeric - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: TCR beta Antibody (H57-597) - Chimeric - Azide and BSA Free [NBP3-20118] -

Immunocytochemistry/Immunofluorescence: TCR beta Antibody (H57-597) - Chimeric - Azide and BSA Free [NBP3-20118] - Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of H57-597 (NBP3-20118) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-20118, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.
TCR beta Antibody (H57-597) - Chimeric - Azide and BSA Free

Flow Cytometry: TCR beta Antibody (H57-597) - Chimeric - Azide and BSA Free [NBP3-20118] -

Flow Cytometry: TCR beta Antibody (H57-597) - Chimeric - Azide and BSA Free [NBP3-20118] - Paraformaldehyde fixed mouse splenocytes were stained with anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG version of H57-597 (NBP3-20118, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Applications for TCR beta Antibody (H57-597) - Chimeric - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This chimeric rabbit antibody was made using the variable domain sequences of the original Hamster IgG format, for improved compatibility with existing reagents, assays and techniques. In an immobilised form, this antibody can directly activate T cells bearing alpha-beta TCR to produce IL-2.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.

Background: TCR beta

Alternate Names

T cell receptor beta chain, TCRB, TCRbeta, Tib

Gene Symbol

Tcrb

Additional TCR beta Products

Product Documents for TCR beta Antibody (H57-597) - Chimeric - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for TCR beta Antibody (H57-597) - Chimeric - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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