TCR beta Antibody (H57-597) - Chimeric - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-20118
Recombinant Monoclonal Antibody
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Mouse
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Lambda Clone # H57-597
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Summary for TCR beta Antibody (H57-597) - Chimeric - Azide and BSA Free
Immunogen
Hamsters were immunised with affinity purified DO-11.10 TCR, and produced detectable anti-TCR antibodies which were judged by the ability of the anti-sera to immunoprecipitate TCR from surface labelled DO-11.10 T cell hybridomas. Hamster 57 was selected for hybridisation, and B cell hybridomas were produced using polyethylene glycol fusion of hamster splenic B cells to the mouse myeloma variant. Selected mAb were screened after subsequent culturing for their ability to immunoprecipitate TCR from detergent lysates of surface-radiolabelled murine T cell hybridomas.
Epitope
H57-597 is directed to an epitope within the FG loop of the C region of TCR beta chain.
Specificity
mAb H57-597 reacts with surface receptors on all alpha-beta TCR bearing cells, but does not react with receptors on gamma-delta T cells.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Lambda
Scientific Data Images for TCR beta Antibody (H57-597) - Chimeric - Azide and BSA Free
Immunocytochemistry/Immunofluorescence: TCR beta Antibody (H57-597) - Chimeric - Azide and BSA Free [NBP3-20118] -
Immunocytochemistry/Immunofluorescence: TCR beta Antibody (H57-597) - Chimeric - Azide and BSA Free [NBP3-20118] - Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of H57-597 (NBP3-20118) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-20118, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.
Flow Cytometry: TCR beta Antibody (H57-597) - Chimeric - Azide and BSA Free [NBP3-20118] -
Flow Cytometry: TCR beta Antibody (H57-597) - Chimeric - Azide and BSA Free [NBP3-20118] - Paraformaldehyde fixed mouse splenocytes were stained with anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG version of H57-597 (NBP3-20118, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.Applications for TCR beta Antibody (H57-597) - Chimeric - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunoprecipitation
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This chimeric rabbit antibody was made using the variable domain sequences of the original Hamster IgG format, for improved compatibility with existing reagents, assays and techniques. In an immobilised form, this antibody can directly activate T cells bearing alpha-beta TCR to produce IL-2.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
0.02% Proclin 300
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
Background: TCR beta
Alternate Names
T cell receptor beta chain, TCRB, TCRbeta, Tib
Gene Symbol
Tcrb
Additional TCR beta Products
Product Documents for TCR beta Antibody (H57-597) - Chimeric - Azide and BSA Free
Product Specific Notices for TCR beta Antibody (H57-597) - Chimeric - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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