Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all TDG Antibodies »

Product Summary for TDG Antibody

Immunogen

The immunogen this antibody was made to, maps to a region between residue 360 to 410 of human Thymine-DNA Glycosylase using the numbering given in entry NP_003202.3 (GeneID 6996).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TDG Antibody

Western Blot: TDG Antibody [NBP2-32248]

Western Blot: TDG Antibody [NBP2-32248]

Western Blot: TDG Antibody [NBP2-32248] - Samples: Whole cell lysate (50 ug) prepared using NETN buffer from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-Thymine DNA glycosylase antibody NBP2-32248 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunohistochemistry-Paraffin: TDG Antibody [NBP2-32248]

Immunohistochemistry-Paraffin: TDG Antibody [NBP2-32248]

Immunohistochemistry-Paraffin: TDG Antibody [NBP2-32248] - FFPE section of human lung cancer. Antibody: Affinity purified rabbit anti- TDG used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Immunohistochemistry-Paraffin: TDG Antibody [NBP2-32248]

Immunohistochemistry-Paraffin: TDG Antibody [NBP2-32248]

Immunohistochemistry-Paraffin: TDG Antibody [NBP2-32248] - FFPE section of human mammary glands. Antibody: Affinity purified rabbit anti- TDG used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
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Applications for TDG Antibody

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:2000

Immunohistochemistry-Paraffin

1:500 - 1:2000

Immunoprecipitation

2 - 10 ug/mg lysate

Western Blot

1:2000 - 1:10000
Application Notes
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: TDG

Thymine DNA glycosylase is encoded by this gene belongs to the TDG/mug DNA glycosylase family. Thymine-DNA glycosylase (TDG) removes thymine moieties from G/T mismatches by hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of DNA and the mispaired thymine. With lower activity, this enzyme also removes thymine from C/T and T/T mispairings. TDG can also remove uracil and 5-bromouracil from mispairings with guanine. This enzyme plays a central role in cellular defense against genetic mutation caused by the spontaneous deamination of 5-methylcytosine and cytosine. This gene may have a pseudogene in the p arm of chromosome 12. [provided by RefSeq]

Long Name

Thymine DNA Glycosylase

Alternate Names

EC 3.2.2.29, thymine-DNA glycosylaseG/T mismatch-specific thymine DNA glycosylase

Gene Symbol

TDG

UniProt

NP_003202.3 (Human)

Additional TDG Products

Product Documents for TDG Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for TDG Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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